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MABF936 Anti-Regnase-1/Zc3h12a Antibody, clone 15D11

This Anti-Regnase-1/Zc3h12a Antibody, clone 15D11 is validated for use in Western Blotting for the detection of Regnase-1/Zc3h12a.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: Zc3h12a, Mcpip, Mcpip1 UniProt Number: Q5D1E7
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MABF936
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWBRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF936
      DescriptionAnti-Regnase-1/Zc3h12a Antibody, clone 15D11
      Alternate Names
      • Ribonuclease ZC3H12A
      • MCP-induced protein 1
      • Regnase-1
      • Zinc finger CCCH domain-containing protein 12A
      Background InformationRibonuclease ZC3H12A (UniProt Q5D1E7; also known as MCP-induced protein 1, Regnase-1, Zinc finger CCCH domain-containing protein 12A) is encoded by the Zc3h12a (also known as Mcpip, Mcpip1) gene (Gene ID 230738) in murine species. Regnase-1 is an endonuclease that is involved in post-transcriptional gene regulation by cleaving target mRNAs or the terminal loop of pre-microRNAs. Regnase-1 negatively regulates Il6, Il12b, Il2, Icos, Ox40, Ctla4, cRel mRNAs, as well as its own mRNA (Zc3h12a). In T cells, regnase-1 and an RNA-binding protein roquin act together to inhibit T helper (TH) cell differentiation by negatively regulating mRNAs encoding the TH17 cell-promoting factors IL-6, ICOS, c-Rel, IRF4, IκBNS and IκBζ. This cooperation requires RNA-binding activity of roquin and the endonuclease activity of regnase-1. Upon T-cell receptor (TCR) activation following antigen binding, however, both roquin and regnase-1 are cleaved and inactivated by the paracaspase MALT1, allowing TH17 differentiation to proceed.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Regnase-1/Zc3h12a Antibody, clone 15D11 is validated for use in Western Blotting for the detection of Regnase-1/Zc3h12a.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected regnase-1 in lysates from wild-type, but not Zc3h12a-/-, mouse embryonic fibroblasts/MEFs (Jeltsch, K.M., et al. (2014). Nat. Immunol. 15(11):1079-1089).
      Biological Information
      ImmunogenRecombinant full-length mouse regnase-1.
      Clone15D11
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 15D11 detected regnase-1 in wild-type, but not Zc3h12a-/-, mouse embryonic fibroblasts/MEFs (Jeltsch, K.M., et al. (2014). Nat. Immunol. 15(11):1079-1089).
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Zc3h12a
      • Mcpip
      • Mcpip1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~70 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MEF1 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Regnase-1/Zc3h12a in 10 µg of MEF1 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF936 04055977312904

      Documentation

      Anti-Regnase-1/Zc3h12a Antibody, clone 15D11 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Regnase-1/Zc3h12a Antibody, clone 15D11 Certificates of Analysis

      TitleLot Number
      Anti-Regnase-1/Zc3h12a, clone 15D11 - 3164797 3164797
      Anti-Regnase-1/Zc3h12a, clone 15D11 - 3378595 3378595
      Anti-Regnase-1/Zc3h12a, clone 15D11 - 3808637 3808637
      Anti-Regnase-1/Zc3h12a, clone 15D11 - 3942729 3942729
      Anti-Regnase-1/Zc3h12a, clone 15D11 - 4187027 4187027
      Anti-Regnase-1/Zc3h12a, clone 15D11 -Q2640165 Q2640165