573822 Anti-Synaptophysin Mouse mAb (SP15)

573822
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
B, Fe, Ht, H, M, Porcine, RMMonoclonal Antibody

Pricing & Availability

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573822-50ULCN
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      Description
      OverviewRecognizes the ~38 kDa synaptophysin protein in brain tissue. Does not cross-react with the ~65 kDa form in mouse liver extracts.
      Catalogue Number573822
      Brand Family Calbiochem®
      Application Data
      Detection of rat synaptophysin by immunoblotting. Sample: Whole tissue extract from rat liver. Primary antibody: Anti-Synaptophysin Mouse mAb (SP15) (Cat. No. 573822) (1:10,000). Detection: chemiluminescence.
      References
      ReferencesAlder, J., et al. 1995. J. Neurosci. 15, 511.
      Thomas, L., et al. 1988. Science 242, 1050.
      Rehmm, H., et al. 1986. EMBO J. 5, 5353.
      Product Information
      FormLiquid
      FormulationIn PBS, 50% glycerol, pH 7.2.
      Negative controlMouse liver extract
      Positive controlRat liver tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesFrozen Sections Honer, W.G., et al. 1993. Brain Res. 609, 9.
      Key Applications Immunoprecipitation
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesFrozen Sections (1:10; see application references)
      Immunoblotting (1:2000, colorimetric)
      Immunocytochemistry (1:250)
      Immunoprecipitation (1:100)
      Application CommentsVariables associated with assay conditions will dictate the proper working dilutions.
      Biological Information
      Immunogensynaptic vesicle-containing fractions from human brain homogenates
      ImmunogenHuman
      CloneSP15
      HostMouse
      IsotypeIgM
      Species Reactivity
      • Bovine
      • Feline
      • Hamster
      • Human
      • Mouse
      • Porcine
      • Rat
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Synaptophysin Mouse mAb (SP15) Certificates of Analysis

      TitleLot Number
      573822

      References

      Reference overview
      Alder, J., et al. 1995. J. Neurosci. 15, 511.
      Thomas, L., et al. 1988. Science 242, 1050.
      Rehmm, H., et al. 1986. EMBO J. 5, 5353.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-February-2008 JSW
      ApplicationFrozen Sections (1:10; see application references)
      Immunoblotting (1:2000, colorimetric)
      Immunocytochemistry (1:250)
      Immunoprecipitation (1:100)
      Application Data
      Detection of rat synaptophysin by immunoblotting. Sample: Whole tissue extract from rat liver. Primary antibody: Anti-Synaptophysin Mouse mAb (SP15) (Cat. No. 573822) (1:10,000). Detection: chemiluminescence.
      DescriptionAmmonium sulfate purified (50%) mouse monoclonal antibody. Recognizes the ~38 kDa syaptophysin protein.
      BackgroundSynaptophysin is a ~38 kDa integral membrane protein on synaptic vesicles. This protein consists of four transmembrane domains, with its amino- and carboxyl-terminus facing the cytoplasm. Due to its transmembrane domain structure, synaptophysin has been postulated to be a gap junction-like protein as voltage-dependent channel activity is seen when synaptophysin hexamers are reconstituted into lipid membranes. Physiological evidence for synaptophysin’s role in secretion includes its involvement in calcium-dependent glutamate secretion in Xenopus oocytes injected with rat cerebellum mRNA.
      HostMouse
      Immunogen speciesHuman
      Immunogensynaptic vesicle-containing fractions from human brain homogenates
      CloneSP15
      IsotypeIgM
      Speciesbovine, feline, hamster, human, mouse, porcine, rat
      Positive controlRat liver tissue
      Negative controlMouse liver extract
      FormLiquid
      FormulationIn PBS, 50% glycerol, pH 7.2.
      Preservative≤0.1% sodium azide
      CommentsVariables associated with assay conditions will dictate the proper working dilutions.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesAlder, J., et al. 1995. J. Neurosci. 15, 511.
      Thomas, L., et al. 1988. Science 242, 1050.
      Rehmm, H., et al. 1986. EMBO J. 5, 5353.
      Application referencesFrozen Sections Honer, W.G., et al. 1993. Brain Res. 609, 9.