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MAB1676-C Anti-Talin 1 Antibody, clone TA205, N-Term, Ascites Free

This Anti-Talin 1 Antibody, clone TA205, N-Term, Ascites Free is validated for use in western blotting, IHC (Paraffin), IP, inhibition & immunofluorescence for the detection of Talin 1.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: TLN1, TLN UniProt Number: Q9Y490
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MAB1676-C
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P), IP, Inhibition, IFMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB1676-C
      DescriptionAnti-Talin 1 Antibody, clone TA205, N-Term, Ascites Free
      Alternate Names
      • Talin-1
      Background InformationTalin 1, also known as Talin 1, and encoded by the gene TLN1/KIAA1027, a large connecting protein located on the cytoplasmic side of most cellular projections. Talin appears to act as an anchoring protein connecting the cytoskeletal protein structures to the plasma membrane. Talin gets concentrated at regions of cell-substratum contact and in particular cells such as lymphocytes at regions of direct cell-cell contact. . Talin 1 binds with high affinity to vinculin and interacts with other proteins found in cellular projections including focal adhesion kinases (FAK) and intermediate filament proteins such as synemin and layilin. Additionally, Talin 1 because of its connections with cytoskeletal proteins talin is associated with many vital cell motion/growth processes from axon guidance to cell migration, muscle contraction, cell-cell interactions, immune responses and platelet activation to name but a few.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Talin 1 Antibody, clone TA205, N-Term, Ascites Free is validated for use in western blotting, IHC (Paraffin), IP, inhibition & immunofluorescence for the detection of Talin 1.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Inhibition
      • Immunofluorescence
      Application NotesImmunohistochemistry Analysis: A 1:2,000 and a 1:500 dilution from a representative lot detected Talin 1 in human prostate cancer sections and human lung tissue, respectively.

      Immunohistochemistry Analysis: A representative lot detected Talin 1 in MeOH fixed fibroblasts.

      Inhibition Analysis: A representative lot from an independent laboratory disrupts actin stress fibers and focal adhesions, and inhibits cell motility when microinjected into human fibroblasts (Bolton, S. J., et al. (1997). Cell Motil Cytoskeleton. 36(4): 363-376.).

      Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Talin 1 from shCTRL and shLRP-1-c1 carcinoma cell lysates (Dedieu, S., et al. (2008). Mol Cell Biol. 28(9):2980-2995.).

      Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Talin 1 from shCTRL and shLRP-1 cell lysates (Langlois, B., et al. (2010). PLoS One. 5(7):e11584.).

      Immunoprecipitation/Western Blotting Analysis: A representative lot from an independent laboratory immunoprecipitated Talin 1 from PC3-2 cell lysates. Immunoprecipitated Talin 1 was then detected in Western Blotting (Trerotola, M. et al. (2012). J Cell Physiol. 227(11): 3670-3676.).

      Immunofluorescence Analysis: A representative lot from an independent laboratory detected Talin 1 in macrophages (Love, D. C., et al. (1998). Exp Parasitol. 88(3):161-171.).

      Immunofluorescence Analysis: A representative lot from an independent laboratory determined that the level of disruption in talin-containing focal complexes is inhibited in LRP-1-silenced carcinoma cells (Dedieu, S., et al. (2008). Mol Cell Biol. 28(9):2980-2995.).

      Immunofluorescence Analysis: A representative lot from an independent laboratory detected Talin-1 in shCTRL and shLRP-1 transfected cells (Langlois, B., et al. (2010). PLoS One. 5(7):e11584.).
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the N-terminus of human Talin 1.
      EpitopeN-terminus
      CloneTA205
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Rabbit based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TLN1
      • TLN
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~270 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Talin 1 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB1676-C 04053252965289

      Documentation

      Anti-Talin 1 Antibody, clone TA205, N-Term, Ascites Free SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Talin 1 Antibody, clone TA205, N-Term, Ascites Free Certificates of Analysis

      TitleLot Number
      Anti-Talin 1, clone TA205, N-Term, Ascites Free - Q2395228 Q2395228
      Anti-Talin 1, clone TA205, N-Term, Ascites Free - 3218875 3218875
      Anti-Talin 1, clone TA205, N-Term, Ascites Free - 3526760 3526760
      Anti-Talin 1, clone TA205, N-Term, Ascites Free - 3745484 3745484
      Anti-Talin 1, clone TA205, N-Term, Ascites Free -2740602 2740602
      Anti-Talin 1, clone TA205, N-Term, Ascites Free Monoclonal Antibody 3008135

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies