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07-2101 Anti-phospho PIX Antibody (Ser340)

This phospho PIX antibody is validated for use in WB for the detection of the phospho PIX protein.

Anti-phospho PIX Antibody (Ser340) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: G-proteins Gene Symbol: PIXB, COOL1, P50BP, BETA-PIX, p85, PAK3, Beta-Pix, P85COOL1, COOL-1, P85, PAK3BP, P50 UniProt Number: Q9ES28
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07-2101
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-2101
      DescriptionAnti-phospho PIX Antibody (Ser340)
      Alternate Names
      • Rho guanine nucleotide exchange factor (GEF) 7
      • PAK-interacting exchange factor beta
      • guanine nucleotide exchange factor 7
      • rho guanine nucleotide exchange factor 7
      • SH3 domain-containing proline-rich protein
      • p85
      • PAK3
      • Beta-Pix
      • P85COOL1
      • COOL-1
      • P85
      • PAK3BP
      • P50
      Background Informationp-21-activated kinase (PAK) interacting exchange factor (PIX) is a guanine nucleotide exchange factor. PIX is a necessary component for the recruitment, regulation and activation of Rac1, as it is able to directly bind Rac1 via its SH3 domain. Studies indicate that PIX may also have an active role as a positive regulator of apoptosis, an inducer of membrane ruffling and in cell migration. Along with Rac1, PIX has been shown to regulate and interact with GIT1 and NOD2. Alternative splicing and initiation give rise to 8 known isoforms. Expression of PIX has been localized to the central nervous system. Isoforms B, C and E have highest expression in the testis and brain.
      References
      Product Information
      FormatAffinity Purified
      Control
      • PIX transfected cell lysates
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide, 1 mg/mL BSA and 30% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationThis phospho PIX antibody is validated for use in WB for the detection of the phospho PIX protein.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to mouse PIX phosphorylated at Ser340.
      EpitopePhosphorylated Ser340
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes PIX phosphorylated at Ser340.
      IsotypeIgG
      Species Reactivity
      • Mouse
      Species Reactivity NoteDemonstrated to react with mouse. Predicted to react with human and rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PIXB
      • COOL1
      • P50BP
      • BETA-PIX
      • p85
      • PAK3
      • Beta-Pix
      • P85COOL1
      • COOL-1
      • P85
      • PAK3BP
      • P50
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. May function as a positive regulator of apoptosis. May function in cell migration (by similarity).

      SUBUNIT STRUCTURE: Interacts with PAK kinases through the SH3 domain. Interacts with unphosphorylated PAK1. Interacts with ITCH. Interacts with SCRIB; interaction is direct and may play a role in regulation of apoptosis by similarity. Interacts with GIT1 and TGFB1I1. Interacts with FRMPD4 (via N-terminus) (by similarity).

      TISSUE SPECIFICITY: Seems to be expressed in the central nervous system. Isoform B, isoform C and isoform E are expressed with highest levels in brain and testis.

      SEQUENCE SIMILARITIES: Contains 1 CH (calponin-homology) domain.

      Contains 1 DH (DBL-homology) domain.

      Contains 1 PH domain.

      Contains 1 SH3 domain.
      Molecular Weight~ 75 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in PIX transfected cell lysates.

      Western Blot Analysis: A 1:1000 dilution of this antibody detected PIX on 10 µg of Pix transfected cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      07-2101 04053252745287

      Documentation

      Anti-phospho PIX Antibody (Ser340) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho PIX Antibody (Ser340) Certificates of Analysis

      TitleLot Number
      Anti-phospho PIX (Ser340) 2898381
      Anti-phospho PIX (Ser340) - 2436781 2436781
      Anti-phospho PIX (Ser340) - 2006938 2006938
      Anti-phospho PIX (Ser340) - 3071952 3071952
      Anti-phospho PIX (Ser340) - 3234493 3234493
      Anti-phospho PIX (Ser340) - 3316685 3316685
      Anti-phospho PIX (Ser340) - 3746896 3746896
      Anti-phospho PIX (Ser340) - NRG1736359 NRG1736359
      Anti-phospho PIX (Ser340) -2826048 2826048
      Anti-phospho PIX (Ser340) Polyclonal Antibody 2967235
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