69041 B834(DE3) Competent Cells - Novagen

69041
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      Plastic ampoule 0.4 ml
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      Description
      OverviewB834 is the parental strain for BL21 (1). These protease-deficient hosts are methionine auxotrophs and allow high specific-activity labeling of target proteins with 35S-methionine and selenomethionine for crystallography (2).

      DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.



      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
      Genotype: F- ompT hsdSB(rB- mB-) gal dcm met (DE3)


      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number69041
      Brand Family Novagen®
      References
      References1. Wood, W. B. (1966) J. Mol. Biol. 16, 118–133.

      2. Leahy, D. J., Hendrickson, W. A., Aukhil, I., and Erickson, H. P. (1992) Science 258, 987–991.
      Product Information
      Components
      Guaranteed efficiencyGuaranteed efficiency: > 2 x 10⁶ cfu/μg
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
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      Supplemental Information
      Specifications

      Documentation

      B834(DE3) Competent Cells - Novagen SDS

      Title

      Safety Data Sheet (SDS) 

      B834(DE3) Competent Cells - Novagen Certificates of Analysis

      TitleLot Number
      69041

      References

      Reference overview
      1. Wood, W. B. (1966) J. Mol. Biol. 16, 118–133.

      2. Leahy, D. J., Hendrickson, W. A., Aukhil, I., and Erickson, H. P. (1992) Science 258, 987–991.

      Brochure

      Title
      Competent Cell Brochure

      Citations

      Title
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    • Gomis-Ruth, F.-X., et al. 1997. Nature 389, 77.
    • Dyda, F., et al. 1994. Science 266, 1981.