71285 BCA Protein Assay Kit

71285
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71285-3CN
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      Glass bottle 500 assa
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      Description
      OverviewThe BCA protein assay is based on a biuret reaction, which is the reduction of Cu2+ to Cu+ by proteins in an alkaline solution with concentration-dependent detection of the monovalent copper ions. Bicinchoninic acid is a chromogenic reagent that chelates the reduced copper, producing a purple complex with strong absorbance at 562 nm (Smith 1985, Wiechelman 1988). This assay can be used to quantify protein concentration with a wide variety of samples and can be performed in minutes.

      The Novagen® BCA Protein Assay Kit can be used to determine protein concentration in the range of 20-2000 µg/ml in either a standard assay or microassay configuration. Kit components are sufficient to complete 500 standard-size reactions (50 µl protein sample plus 1 ml reagent) or 2500 micro-scale reactions (25 µl protein sample plus 200 µl reagent). A BSA standard (2 mg/ml) is provided for convenient and reliable preparation of standard curves.

      This assay is robust and can be performed in the presence of many compounds. Some reagents, including chelating agents, strong acids or bases, and reducing agents, interfere with the reduction and chelating reactions on which this assay depends (Brown 1989). The BCA assay is compatible with the following Novagen protein extraction and lysis reagents: BugBuster® Protein Extraction Reagent, PopCulture® Reagent, CytoBuster™ Protein Extraction Reagent, Reportasol™ Extraction Buffer, and Insect PopCulture Reagent. Options for the removal or dilution of interfering substances are described in the kit literature.
      Catalogue Number71285
      Brand Family Novagen®
      References
      ReferencesSmith, P.K., et al. 1985. Anal. Biochem. 150, 76.

      Kessler, R. J. and Fanestil, D. D. 1986. Anal. Biochem 159, 138.

      Wiechelman, K., et al. 1988. Anal. Biochem. 175, 231.

      Brown, R., et al. 1989. Anal. Biochem. 180, 136.
      Product Information
      500 mlBCA Solution
      15 ml4% Cupric Sulfate
      3 x 1 mlBSA Standard, 2 mg/ml
      DeclarationUse of the BCA protein assay is permitted for research purposes only.
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage +15°C to +30°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      BCA Protein Assay Kit SDS

      Title

      Safety Data Sheet (SDS) 

      BCA Protein Assay Kit Certificates of Analysis

      TitleLot Number
      71285

      References

      Reference overview
      Smith, P.K., et al. 1985. Anal. Biochem. 150, 76.

      Kessler, R. J. and Fanestil, D. D. 1986. Anal. Biochem 159, 138.

      Wiechelman, K., et al. 1988. Anal. Biochem. 175, 231.

      Brown, R., et al. 1989. Anal. Biochem. 180, 136.

      Citations

      Title
    • Matthias E. Liechti, et al. (2007) Metabotropic glutamate 2/3 receptors in the ventral tegmental area and the nucleus accumbens shell are involved in behaviors relating to nicotine dependence. Journal of Neuroscience 27, 9077-9085.
    • User Protocols

      Title
      TB380 BCA Protein Assay Kit