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SCT215 BioTracker™ 497 Green Actin Live Cell Probe

Live cell imaging cytoskeleton probe used to detect actin using fluorescence microscopy or flow cytometry.

SCT215
5 vials  
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCT215
      DescriptionBioTracker™ 497 Green Actin Live Cell Probe
      OverviewThe BioTracker™ 497 Green Actin Live Cell Probe may be used in both living cells and fixed-cell applications. Because SCT215 is an organic fluorescent small molecule with ready membrane permeability, it enables visualization of intracellular actin filamentsby simple addition of the probe to the cell culture medium or extracellular fluid without a rinsing step.

      This probe is thought to bind to F-actin through the common binding site for phalloidin and jasplakinolide.

      Spectral Properties
      Absorbance (max): 497 nm
      Emission (max): 525 nm (Orange)
      Alternate Names
      • Live Cell Imaging Probe
      • Live Cell Actin Probe
      • Green Live Cell Actin Probe
      Background InformationThe BioTracker™ 497 Green Actin Live Cell Probe is a fluorescent probe that specifically binds to actin filaments (F-actin) and can visualize F-actin with green fluorescence.
      References
      Product Information
      Detection methodFluorescence Flow cytometry
      FormatLyophilized solid
      PresentationLyophilized. Orange solid.
      Quality LevelMQ100
      Applications
      ApplicationLive cell imaging cytoskeleton probe used to detect actin using fluorescence microscopy or flow cytometry.
      Key Applications
      • Cell Based Assays
      • Flow Cytometry
      • Fluorescence
      Application NotesSCT215 fluorescence can
      be observed with a blue excitation
      and green fluorescence filter set for GFP/FITC. For
      live-cell imaging, use lower concentrations of
      probe, weaken the excitation intensity and use
      high-sensitivity cameras to reduce exposure time.
      Consider the use of antifade reagent for time-lapse
      or continuous imaging.
      Stock solution is prepared by adding 30 uL DMSO to one vial,, which results in a pale yellow liquid.
      Diluting stock solution in culture medium to 50-100 nM before adding to cells is recommended, although optimal incubation concentraiton may be determined according to user application. Use the lowest possible concentration to limit cytotoxicity.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore BioTracker™ 497 Green Actin Live Cell Probe at -20°C, desiccated and protected from light. Centrifuge vial briefly to collect contents at bottom of vial before opening. After dissolving in DMSO, store aliquots at -20 °C.
      Packaging Information
      Material Size5 vials
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCT215 04065269233329