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2384 Creatine Phosphokinase, Rabbit Skeletal Muscle

2384
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2384-20MGCN
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      Plastic ampoule 20 mg
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      Description
      OverviewNative creatine phosphokinase from rabbit muscle. Catalyzes the conversion of creatine and ATP to creatine phosphate and ADP. Has an optimal pH of 9.0 for the forward reaction and 6.0-7.0 for the reverse reaction.
      Catalogue Number2384
      Brand Family Calbiochem®
      SynonymsrCPK
      References
      ReferencesBessman, S.P. 1987. Anal. Biochem. 161, 519.
      Product Information
      CAS number9001-15-4
      Activity≥300 units/mg (at 25°C); 750 units/mg (at 37°C)
      Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the conversion of 1.0 µmol creatine phosphate per min at 25°C or 37°C, pH 6.8.
      EC number2.7.3.2
      FormLyophilized
      Hygroscopic Hygroscopic
      Quality LevelMQ200
      Applications
      Biological Information
      Physicochemical Information
      ContaminantsATPase, hexokinase, myokinase: <0.001%
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution in 0.5% NaHCO₃, refrigerate (4°C). Do not freeze or shake, as SH groups may be oxidized and form linkages between protein molecules, resulting in precipitation, turbidity, and loss of activity. NaHCO₃ stock solutions are stable for 2-3 days at 4°C. Following reconstitution in 0.1 M imidazole buffer, aliquot and freeze (-20°C). Imidazole stock solutions are stable for up to 4 weeks at -20°C. Avoid freeze/thaw cycles, as 20-30% of activity will be lost with each freeze/thaw cycle.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Creatine Phosphokinase, Rabbit Skeletal Muscle SDS

      Title

      Safety Data Sheet (SDS) 

      Creatine Phosphokinase, Rabbit Skeletal Muscle Certificates of Analysis

      TitleLot Number
      2384

      References

      Reference overview
      Bessman, S.P. 1987. Anal. Biochem. 161, 519.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-October-2007 JSW
      SynonymsrCPK
      DescriptionNative creatine phosphokinase from rabbit muscle. Catalyzes the reversible conversion of creatine and ATP to creatine phosphate and ADP. Has an optimal pH of 9.0 for the forward reaction and 6.0-7.0 for the reverse reaction.
      FormLyophilized
      CAS number9001-15-4
      EC number2.7.3.2
      ContaminantsATPase, hexokinase, myokinase: <0.001%
      Activity≥300 units/mg (at 25°C); 750 units/mg (at 37°C)
      Unit definitionOne unit is defined as the amount of enzyme that will catalyze the conversion of 1.0 µmol creatine phosphate per min at 25°C or 37°C, pH 6.8.
      Solubility0.5% NaHCO₃, pH 9.0 or 0.1 M Imidazole Buffer, pH 6.6
      Storage -20°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution in 0.5% NaHCO₃, refrigerate (4°C). Do not freeze or shake, as SH groups may be oxidized and form linkages between protein molecules, resulting in precipitation, turbidity, and loss of activity. NaHCO₃ stock solutions are stable for 2-3 days at 4°C. Following reconstitution in 0.1 M imidazole buffer, aliquot and freeze (-20°C). Imidazole stock solutions are stable for up to 4 weeks at -20°C. Avoid freeze/thaw cycles, as 20-30% of activity will be lost with each freeze/thaw cycle.
      Toxicity Standard Handling
      ReferencesBessman, S.P. 1987. Anal. Biochem. 161, 519.