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260913 DNase I, Bovine Pancreas

260913
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260913-25MUCN
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      Plastic ampoule 25 mu
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      Description
      OverviewNative DNase I from bovine pancreas. An endonuclease that preferentially cleaves phosphodiester linkages adjacent to pyrimidine nucleotides to yield 5′-phosphate terminated polynucleotides with a free hydroxyl group at the 3′ position. Acts on single and double stranded DNA. Note: 1 MU = 1,000,000 Dornase units
      Note: 32 Dornase units equal ~1 Kunitz unit; 1 MU = 1,000,000 Dornase units.
      Catalogue Number260913
      Brand Family Calbiochem®
      SynonymsDeoxyribonuclease I
      References
      ReferencesTakeshita, H., et al. 2000. Biochem. Biophys. Res. Commun. 269, 481.
      Jones, S.J., et al. 1996. J. Mol. Biol. 264, 1154.
      Product Information
      CAS number9003-98-9
      Activity≥60,000 Dornase units/mg dry weight.
      Unit of DefinitionOne Dornase unit is defined as the amount of enzyme that will cause a decrease of 1.0 relative viscosity unit in a solution of highly polymerized DNA from the initial relative viscosity of 4.0 in 10 min at 30°C.
      EC number3.1.21.1
      FormLyophilized
      Hygroscopic Hygroscopic
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSRF0750000
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Moisture Protect from moisture
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      DNase I, Bovine Pancreas Certificates of Analysis

      TitleLot Number
      260913

      References

      Reference overview
      Takeshita, H., et al. 2000. Biochem. Biophys. Res. Commun. 269, 481.
      Jones, S.J., et al. 1996. J. Mol. Biol. 264, 1154.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision30-April-2008 RFH
      SynonymsDeoxyribonuclease I
      DescriptionNative DNase I from bovine pancreas. An endonuclease that preferentially cleaves phosphodiester linkages adjacent to pyrimidine nucleotides to yield 5′-phosphate terminated polynucleotides with a free hydroxyl group at the 3′ position. Acts on single and double stranded DNA. Note: 1 MU = 1,000,000 Dornase units
      FormLyophilized
      CAS number9003-98-9
      RTECSRF0750000
      EC number3.1.21.1
      Activity≥60,000 Dornase units/mg dry weight.
      Unit definitionOne Dornase unit is defined as the amount of enzyme that will cause a decrease of 1.0 relative viscosity unit in a solution of highly polymerized DNA from the initial relative viscosity of 4.0 in 10 min at 30°C.
      SolubilitySodium Acetate Buffer or H₂O
      Storage -20°C
      Hygroscopic
      Protect from moisture
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      Merck USA index14, 2906
      ReferencesTakeshita, H., et al. 2000. Biochem. Biophys. Res. Commun. 269, 481.
      Jones, S.J., et al. 1996. J. Mol. Biol. 264, 1154.