SCR128 Dopaminergic Differentiation Growth Factor Sampler

SCR128
1 Kit  
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      Overview

      Replacement Information

      Key Spec Table

      SourcePurity
      E. coli.Recombinant growth factors are endotoxin free (<0.1 ng/μg of protein (<1 EU/μg) and greater than 98% pure by SDS-PAGE gel and HPLC analyses.
      Description
      Catalogue NumberSCR128
      DescriptionDopaminergic Differentiation Growth Factor Sampler
      Background InformationEMD Millipore’s Dopaminergic Differentiation Growth Factor Sampler contains a collection of five validated growth factors that are routinely used to induce differentiation of human pluripotent ES/iPS cells to dopaminergic neurons along with a reliable high affinity antibody marker, anti-tyrosine hydrodylase, to aid in quantifying the percentage yields from differentiation experiments.
      References
      Product Information
      Components
      • 1. Sonic HedgeHog (Shh), Human Recombinant (Part No. GF174-5UG): Two 5 μg vials. Lyophilized powder. Store at -20°C.
      • 2. Fibroblast Growth Factor-8 (FGF-8), Human Recombinant (Part No. GF110-5UG): One 5 μg vial. Lyophilized powder. Store at -20°C.
      • 3. Brain Derived Neurotrophic Factor (BDNF), Human Recombinant (Part No. GF029-2UG): One 2 μg vial. Lyophilized powder. Store at -20°C.
      • 4. Glial Derived Neurotrophic Factor (GDNF), Human Recombinant (Part No. GF030-2UG): One 2 μg vial. Lyophilized powder. Store at -20°C.
      • 5. Transforming Growth Factor β-III (TGF-β-III), Human Recombinant (Part No. GF176-2UG): One 2 μg vial. Lyophilized powder. Store at -20°C.
      • 6. Anti-Tyrosine Hydroxylase antibody, rabbit polyclonal (Part No. AB152-20): One 20 μL vial. Store at -20°C.
      Quality LevelMQ100
      Applications
      ApplicationThis Dopaminergic Differentiation Growth Factor Sampler contains five validated growth factors used to induce differentiation of human pluripotent Embryonic stem (ES) & induced pluripotent Stem (iPS) cells to dopaminergic neurons.
      Biological Information
      PurityRecombinant growth factors are endotoxin free (<0.1 ng/μg of protein (<1 EU/μg) and greater than 98% pure by SDS-PAGE gel and HPLC analyses.
      SourceE. coli.
      Cross ReactivityNone
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceUsing the growth factors supplied in this kit a 3-5 fold increase of dopaminergic differention (up to 50-70% TH+ cells) was routinely produced using HES/hips cell derived neural progenitor cells vs. spontaneous neuronal differentiation.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20°C for up to 6 months from date of receipt.
      Packaging Information
      Material Size1 Kit
      Transport Information
      Supplemental Information
      Specifications