344905 FURA 2/AM - CAS 108964-32-5 - Calbiochem

344905
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344905-1MGCN
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      Description
      OverviewCell-permeable ester form of FURA 2. Note: 1 set = 10 x 100 µg.
      PLURONIC® F-127, PROTEIN GRADE® Detergent (Cat. No. 540025, available separately) is a mild, low-toxicity detergent that can aid in the solubilization of FURA 2/AM.
      Catalogue Number344905
      Brand Family Calbiochem®
      Synonyms1-[2-(5-Carboxyoxazol-2-yl)-6-aminobenzofuran-5-oxy]-2-(2ʹ-amino-5ʹ-methylphenoxy)-ethane-N,N,NʹNʹ-tetraacetic Acid Pentaacetoxymethyl Ester
      References
      ReferencesDavis, M.J., et al. 1992. Am. J. Physiol. 263, H1292.
      Rao, G.H., et al. 1992. Biochem. Med. Metab. Biol. 47, 226.
      Schwartz, Y., et al. 1992. Endocrinology 131, 772.
      Ward, C.A., and Moffat, M.P. 1992. J. Mol. Cell. Cardiol. 24, 937.
      Janconi, M.E., et al. 1990. J. Cell Biol. 110, 1555.
      Tsien, R.Y. 1980. Biochemistry 19, 2396.
      Product Information
      CAS number108964-32-5
      ATP CompetitiveN
      FormYellow solid
      Hill FormulaC₄₄H₄₇N₃O₂₄
      Chemical formulaC₄₄H₄₇N₃O₂₄
      ReversibleN
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetRatiometric calcium indicator
      Purity≥95% by HPLC.
      Physicochemical Information
      Cell permeableY
      Emission max.
      Excitation max.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) under an inert gas. Stock solutions are stable for up to 1 month at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      FURA 2/AM - CAS 108964-32-5 - Calbiochem Certificates of Analysis

      TitleLot Number
      344905

      References

      Reference overview
      Davis, M.J., et al. 1992. Am. J. Physiol. 263, H1292.
      Rao, G.H., et al. 1992. Biochem. Med. Metab. Biol. 47, 226.
      Schwartz, Y., et al. 1992. Endocrinology 131, 772.
      Ward, C.A., and Moffat, M.P. 1992. J. Mol. Cell. Cardiol. 24, 937.
      Janconi, M.E., et al. 1990. J. Cell Biol. 110, 1555.
      Tsien, R.Y. 1980. Biochemistry 19, 2396.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Fluorescent Calcium Probes Technical Bulletin
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-June-2008 RFH
      Synonyms1-[2-(5-Carboxyoxazol-2-yl)-6-aminobenzofuran-5-oxy]-2-(2ʹ-amino-5ʹ-methylphenoxy)-ethane-N,N,NʹNʹ-tetraacetic Acid Pentaacetoxymethyl Ester
      DescriptionCell permeant ester form of FURA 2 (Cat. No. 344900). The active dye is rapidly released in the cell by cytoplasmic esterases. Fluorescent calcium chelator (Kd = 224 nM) that offers 30 times greater fluorescence intensity than QUIN 2 (Cat. No. 551826). Can be used as a probe in many cellular systems, including cardiac myocytes, vascular smooth muscle cells, and medullary collecting tubules. Spectral properties in the presence of low calcium concentrations (excitation max.: 362 nm, emission max.: 512 nm) differ from those during high calcium conditions (excitation max.: 335 nm, emission max.: 505 nm). Note: 1 set = 10 x 100 µg.
      FormYellow solid
      Intert gas (Yes/No) Packaged under inert gas
      CAS number108964-32-5
      Chemical formulaC₄₄H₄₇N₃O₂₄
      Structure formulaStructure formula
      Purity≥95% by HPLC.
      SolubilityDMSO (10 mg/ml). Further dilute with aqueous buffers just prior to use.
      Storage -20°C
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) under an inert gas. Stock solutions are stable for up to 1 month at -20°C.
      Toxicity Standard Handling
      ReferencesDavis, M.J., et al. 1992. Am. J. Physiol. 263, H1292.
      Rao, G.H., et al. 1992. Biochem. Med. Metab. Biol. 47, 226.
      Schwartz, Y., et al. 1992. Endocrinology 131, 772.
      Ward, C.A., and Moffat, M.P. 1992. J. Mol. Cell. Cardiol. 24, 937.
      Janconi, M.E., et al. 1990. J. Cell Biol. 110, 1555.
      Tsien, R.Y. 1980. Biochemistry 19, 2396.