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109204 Giemsa's azur eosin methylene blue solution

for microscopy

Giemsa's azur eosin methylene blue solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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109204
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      		 Barrel PE/met 25 l
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      Description
      Catalogue Number109204
      DescriptionGiemsa's azur-eosin-methylene blue solution
      OverviewGiemsa’s Azure Eosin Methylene Blue solution - for microscopy, can be used for the staining of blood and bone marrow smears, paraffin sections and clinical-cytological specimens. Giemsa staining is one of the standard procedures in histology, demonstrating various cells with their morphological features better than with H&E stain. When used in hematological applications, Giemsa’s stain is frequently used in combination with other staining solutions, e. g. with May-Grünwald’s solution for Pappenheim (MGG) overview staining. This ready-to-use staining solution generally stains the nuclei red, based on the molecular interaction between the eosin Y dye and an Azure B-DNA complex. Both dyes assemble to an Eosin Y - Azure B-DNA complex and the intensity of the resulting stain depends on the content of Azure B and the ratio of Azure B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances. In histology and clinico-cytological applications, Giemsa’s staining without additional stainings is used as an extended overview staining method. In this method, the color of the various cell components is influenced by pretreatment of the specimen material. Here, chromatin-containing structures (e. g. cell nuclei) appear in various blue shades, while the acidophilic components show up in a variety of red shades. The 500 ml bottle is sufficient for 3500-5000 stainings. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
      References
      Product Information
      HS Code3212 90 00
      Quality LevelMQ400
      Applications
      Applicationfor microscopy
      Biological Information
      Physicochemical Information
      Boiling point>65 °C (1013 hPa)
      Density0.99 g/cm3 (20 °C)
      Explosion limit5.5 - 44 %(V) Methanol
      Flash point18 °C
      Ignition temperature455 °C
      pH value6.0 - 8.2 (H₂O, 20 °C) (undiluted)
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Hazard Pictogram(s) Hazard Pictogram(s)
      Hazard Pictogram(s)
      Hazard Pictogram(s)
      Hazard Statement(s)H225: Highly flammable liquid and vapour.
      H301 + H311 + H331: Toxic if swallowed, in contact with skin or if inhaled.
      H317: May cause an allergic skin reaction.
      H370: Causes damage to organs.
      Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
      P233: Keep container tightly closed.
      P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection.
      P301 + P310: IF SWALLOWED: Immediately call a POISON CENTER/doctor.
      P303 + P361 + P353: IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water.
      P304 + P340 + P311: IF INHALED: Remove person to fresh air and keep comfortable for breathing. Call a POISON CENTER/doctor.
      P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
      Signal WordDanger
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Packaging Information
      Transport Information
      Declaration (railroad and road) ADR, RIDUN 1230 , 3 (6.1), II
      Declaration (transport by air) IATA-DGRUN 1230 , 3 (6.1), II
      Declaration (transport by sea) IMDG-CodeUN 1230 , 3 (6.1), II
      Supplemental Information
      Specifications
      Suitability for microscopypasses test
      Erythrocytesreddish-brownish
      Nuclei / Chromatinred to violet
      Eosinophilic granulesreddish to red-brown
      Neutrophilic granuleslight violet
      Lymphocyte cytoplasmnblue
      Monocyte cytoplasmgrey-blue
      Thrombocytesviolet
      Basophilic granulesdark violet
      Nuclei (Tissue section)blue to dark blue
      Cellsblue to dark blue
      Collagenpale blue
      Osteoidpale blue
      Eosinophilic granula (Tissue section)red
      Acidic mucopolysaccharidesreddish-violet
      Mast cell granulesreddish-violet
      Cartilage matrixreddish-violet
      Acidophilic materialsorange-red
      Helicobacter pylori
      blue to dark blue
      blue to dark blue
      Global Trade Item Number
      Catalogue Number GTIN
      1.09204.9025 04022536111940

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