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AP202A Goat Anti-Rat light chain Antibody, Alkaline Phosphatase conjugate

Detect light chain using this Goat anti-Rat light chain Antibody, Alkaline Phosphatase conjugate validated for use in ELISA & WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Species Reactivity: Rat
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AP202A
0.5 mL  
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      Description
      Catalogue NumberAP202A
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionGoat Anti-Rat light chain Antibody, Alkaline Phosphatase conjugate
      Background InformationLight chains covalently bound to a pair of heavy chains to form an antibody molecule. Immunoglobulin light chains occur in two types, kappa or lambda. Light chain's mass is about 25,000 Daltons per mole. Each light chain consists of about 250 amino-acids long. These are usually designated by the Greek letters kappa and lambda. The ratio of kappa to lambda found in the immunoglobulin population varies by species.
      References
      Product Information
      FormatAlkaline Phosphatase
      PresentationPurified by immunoaffinity chromatography. Liquid in 0.01M Tris-HCl, 0.25M NaCl, pH 8.0, 0.05% Sodium Azide. 15 mg/mL BSA as stabilizer.
      Quality LevelMQ100
      Applications
      ApplicationDetect light chain using this Goat anti-Rat light chain Antibody, Alkaline Phosphatase conjugate validated for use in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Application NotesWestern Blotting: 1:5,000 -1:50,000

      ELISA: 1:5,000 -1:50,000

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenPrepared from purified rat IgG light chain.
      EpitopeKappa light chain
      HostGoat
      SpecificityThe antibody reacts strongly with native primary antibodies primarily with kappa light chains. It is not suitable for detecting lambda light chains. The antibody does not react with the heavy chain of rat IgG. The antibody has been tested by ELISA and adsorbed to ensure minimal cross-reaction with bovine, goat, horse, human, mouse, rabbit, and sheep immunoglobulins.
      IsotypeIgG
      Species Reactivity
      • Rat
      Species Reactivity NoteMinimal cross-reaction with bovine, goat, horse, human, mouse, rabbit, and sheep.
      Antibody TypePolyclonal Antibody
      Purification MethodImmunoAffinity Purified
      Molecular Weight25 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized product at 2°-8°C for up to 12 months. After reconstitution the product is stable for six weeks at 2°-8°C in the dark as an undiluted liquid. Reconstitute vial with of distilled water (addition of 0.5 ml water will yield a final concentration of 1 mg/mL). For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze thaw cycles. Keep away from light.
      Packaging Information
      Material Size0.5 mL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AP202A 04053252368073

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      Categories

      Life Science Research > Antibodies and Assays > Secondary Antibodies