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382180 Histone Deacetylase 6, GST-Fusion, Human Recombinant, S. frugiperda

Histone Deacetylase 6, GST-Fusion, Human Recombinant, S. frugiperda, is a full-length HDAC6 fused to GST at the N-terminus. Useful in the study of HDAC6 regulation and for inhibitor screening.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: HDAC6, Human, Recombinant

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382180
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      Description
      OverviewFull length, recombinant, human histone deacetylase 6 (HDAC6) fused to GST at the N-terminus and expressed in S. frugiperda insect cells using a baculovirus expression system. Actively maintained in the cytoplasm, HDAC6 is uniquely dependent on its ubiquitin-binding domain and functions at the nexus between two cellular signaling systems, involving protein acetylation and ubiquitination. The active deacetylase is useful in the study of HDAC6 regulation and for inhibitor screening.
      Catalogue Number382180
      Brand Family Calbiochem®
      SynonymsHDAC6, Human, Recombinant
      References
      ReferencesPandey, U.B., et al. 2007. Nature 447, 859.
      Hubbert, C., et al. 2002. Nature 417, 455.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enyme that is required to deacetylate 1 pmol fluorescent HDAC peptide substrate per min at 30°C.
      FormLiquid
      FormulationIn 138 mM NaCl, 25 mM Tris-HCl, 0.05% Tween®-20 detergent, 10% glycerol, pH 8.0.
      Quality LevelMQ100
      Applications
      ApplicationHistone Deacetylase 6, GST-Fusion, Human Recombinant, S. frugiperda, is a full-length HDAC6 fused to GST at the N-terminus. Useful in the study of HDAC6 regulation and for inhibitor screening.
      Biological Information
      Purity≥80% by SDS-PAGE
      Specific Activity≥50 units/µg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      382180-50UGCN 04055977212822

      Documentation

      Histone Deacetylase 6, GST-Fusion, Human Recombinant, S. frugiperda SDS

      Title

      Safety Data Sheet (SDS) 

      Histone Deacetylase 6, GST-Fusion, Human Recombinant, S. frugiperda Certificates of Analysis

      TitleLot Number
      382180

      References

      Reference overview
      Pandey, U.B., et al. 2007. Nature 447, 859.
      Hubbert, C., et al. 2002. Nature 417, 455.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-August-2008 JSW
      SynonymsHDAC6, Human, Recombinant
      DescriptionFull length, recombinant, human histone deacetylase 6 (HDAC6) fused to GST at the N-terminus and expressed in S. frugiperda insect cells using a baculovirus expression system. Actively maintained in the cytoplasm, HDAC6 is uniquely dependent on its ubiquitin-binding domain and functions at the nexus between two cellular signaling systems, involving protein acetylation and ubiquitination. The active deacetylase is useful in the study of HDAC6 regulation and for inhibitor screening.
      FormLiquid
      FormulationIn 138 mM NaCl, 25 mM Tris-HCl, 0.05% Tween®-20 detergent, 10% glycerol, pH 8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions
      137 mM NaCl, 25 mM Tris-HCl, 2.7 mM KCl, 1 mM MgCl2 0.1 mg/ml BSA, 30 µM fluorescent HDAC substrate, 5 ng/µl HDAC6; incubate at 30° for 60 min.
      Purity≥80% by SDS-PAGE
      Specific activity≥50 units/µg protein
      Unit definitionOne unit is defined as the amount of enyme that is required to deacetylate 1 pmol fluorescent HDAC peptide substrate per min at 30°C.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesPandey, U.B., et al. 2007. Nature 447, 859.
      Hubbert, C., et al. 2002. Nature 417, 455.