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71187 Insect PopCulture® Reagent

Protein extraction directly from insect cell cultures

Insect PopCulture® Reagent MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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      Overview

      Replacement Information

      Insect PopCulture® Reagent is a detergent-based lysis reagent specifically formulated for total insect cell culture extraction without the need for centrifugation. The improved method recovers both protein released into the medium and intracellular protein, increasing processing efficiency and target protein yields (Loomis 2002). It is amenable for automated expression-level screening and is fully compatible with the Ni-NTA His•Bind® affinity purification method. Insect PopCulture reagent can be used for protein extraction from insect cells grown in suspension and adherent cells grown on tissue culture plates.



      Description

      Catalogue Number71187 Brand Family Novagen®
      Features and benefits
      • No need to separate cells from culture media
      • No need to clarify cell extracts prior to purification
      • Higher protein yield due to target protein recovery from both medium and cells
      • Direct affinity adsorption of target proteins to IMAC affinity resin from the total culture extract
      • Compatible with protease inhibitor cocktails
      • Ideal for high-throughput, expression-level screening and protein purification
      • Compatible with transfected and baculovirus-infected insect cell cultures
      References
      ReferencesLoomis, K., et al. 2002. inNovations 15, 16.
      Product Information
      DeclarationThis product is covered by EP Patent 1,432,822 owned by EMD Chemicals Inc. or its Affiliates.
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Irritant
      Storage +15°C to +30°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      71187 0