475989 MTT - CAS 298-93-1 - Calbiochem

475989
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Overview

Replacement Information

Key Spec Table

Empirical FormulaCAS #
C₁₈H₁₆BrN₅S298-93-1

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      Glass bottle 1 gm
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      Description
      OverviewMembrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan. Spectrophotometric measurement of MTT-formazan at 540 or 570 nm allows quantitation of cell viability. Used as a direct indicator of cytotoxicity (such as for screening cancer drugs) and apoptosis. An electron acceptor used for studying NADP-dependent dehydrogenases.
      Catalogue Number475989
      Brand Family Calbiochem®
      Synonyms3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium Bromide, Thiazole Blue
      References
      ReferencesLiu, Y., et al. 1997. J. Neurochem. 69, 581.
      Abdallah, M.A. 1980. Eur. J. Biochem. 112, 331.
      Product Information
      CAS number298-93-1
      ATP CompetitiveN
      FormYellow powder
      Hill FormulaC₁₈H₁₆BrN₅S
      Chemical formulaC₁₈H₁₆BrN₅S
      ReversibleN
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetUsed as a direct indicator of cytotoxicity and apoptosis.
      Purity≥98% by HPLC
      Physicochemical Information
      Cell permeableY
      ContaminantsSulfated ash: ≤0.5%; heavy metals: ≤0.001%
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSXF8060000
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Harmful & Carcinogenic / Teratogenic
      Storage +15°C to +30°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C.). Stock solutions are stable for up to 1 month at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MTT - CAS 298-93-1 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      MTT - CAS 298-93-1 - Calbiochem Certificates of Analysis

      TitleLot Number
      475989

      References

      Reference overview
      Liu, Y., et al. 1997. J. Neurochem. 69, 581.
      Abdallah, M.A. 1980. Eur. J. Biochem. 112, 331.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-December-2019 JSW
      Synonyms3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium Bromide, Thiazole Blue
      DescriptionMembrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan. Spectrophotometric measurement of MTT-formazan at 540 or 570 nm allows quantitation of cell viability. Used as a direct indicator of cytotoxicity (such as for screening cancer drugs) and apoptosis. An electron acceptor used for studying NADP-dependent dehydrogenases.
      FormYellow powder
      CAS number298-93-1
      RTECSXF8060000
      Chemical formulaC₁₈H₁₆BrN₅S
      Structure formulaStructure formula
      Purity≥98% by HPLC
      ContaminantsSulfated ash: ≤0.5%; heavy metals: ≤0.001%
      SolubilityH₂O (5 mg/ml)
      Storage +15°C to +30°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C.). Stock solutions are stable for up to 1 month at -20°C.
      Toxicity Harmful & Carcinogenic / Teratogenic
      ReferencesLiu, Y., et al. 1997. J. Neurochem. 69, 581.
      Abdallah, M.A. 1980. Eur. J. Biochem. 112, 331.