537059 Propidium Iodide - CAS 25535-16-4 - Calbiochem

537059
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Overview

Replacement Information

Key Spec Table

Empirical FormulaCAS #
C₂₇H₃₄N₄I₂25535-16-4

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537059-100MGCN
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      Glass bottle 100 mg
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      Description
      OverviewMembrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for flow cytometry for staining apoptotic cells and nuclei. Can be used to differentiate between apoptotic and necrotic cell death while stains only necrotic cells. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate (FITC).
      Extinction coefficient(493 nm): 5900 M -1cm-1;(287 nm): 55,700 M -1cm-1;
      Catalogue Number537059
      Brand Family Calbiochem®
      References
      ReferencesBelloc, F., et al. 1994. Cytometry 17, 59.
      Crompton, T., et al. 1992. Biochem. Biophys. Res. Commun. 183, 532.
      Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795.
      de Caestecker, M.P., et al. 1992. J. Immunol. Methods 154, 11.
      Pollice, A.A., et al. 1992. Cytometry 13, 432.
      Product Information
      CAS number25535-16-4
      ATP CompetitiveN
      FormDark red solid
      Hill FormulaC₂₇H₃₄N₄I₂
      Chemical formulaC₂₇H₃₄N₄I₂
      ReversibleN
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetMembrane impermeable DNA intercalator
      Sample TypeIntact Cells
      Physicochemical Information
      Cell permeableN
      Emission max.
      Excitation max.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSSF7949600
      Safety Information
      R PhraseR: 36/37/38

      Irritating to eyes, respiratory system and skin.
      S PhraseS: 26

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Irritant
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Propidium Iodide - CAS 25535-16-4 - Calbiochem Certificates of Analysis

      TitleLot Number
      537059

      References

      Reference overview
      Belloc, F., et al. 1994. Cytometry 17, 59.
      Crompton, T., et al. 1992. Biochem. Biophys. Res. Commun. 183, 532.
      Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795.
      de Caestecker, M.P., et al. 1992. J. Immunol. Methods 154, 11.
      Pollice, A.A., et al. 1992. Cytometry 13, 432.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure

      Citations

      Title
    • Oliveira, J.B., et al. 2007. Procedings of the National Academy of Science 104, 8953.
    • Tirado, O.M., et al. 2005. Cancer Research 65, 9320.
    • Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision29-August-2008 RFH
      DescriptionMembrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for staining apoptotic cells and nuclei by flow cytometry. Can be used to differentiate between apoptotic and necrotic cell death. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate. Has the following spectral properties: extinction coefficient: 5,900 M-1cm-1 (493 nm); 55,700 M-1cm-1 (287 nm); 13,800 M-1cm-1 (252.5 nm); excitation maximum: 536 nm; emission maximum: 617 nm.
      FormDark red solid
      CAS number25535-16-4
      RTECSSF7949600
      Chemical formulaC₂₇H₃₄N₄I₂
      Structure formulaStructure formula
      SolubilityH₂O. Solubility may be improved by first dissolving in a very small amount of DMSO, then adding material to an aqueous solution.
      Storage +2°C to +8°C
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Toxicity Irritant
      ReferencesBelloc, F., et al. 1994. Cytometry 17, 59.
      Crompton, T., et al. 1992. Biochem. Biophys. Res. Commun. 183, 532.
      Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795.
      de Caestecker, M.P., et al. 1992. J. Immunol. Methods 154, 11.
      Pollice, A.A., et al. 1992. Cytometry 13, 432.
      Citation
    • Oliveira, J.B., et al. 2007. Procedings of the National Academy of Science 104, 8953.
    • Tirado, O.M., et al. 2005. Cancer Research 65, 9320.