70548 T7Select™10-3b DNA

70548
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70548-3CN
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      Glass bottle 10 μg
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      Description
      OverviewThe T7Select™10-3b vector is a protein display vector based on bacteriophage T7. The vector displays an average of 5–15 copies of peptides and proteins up to 1200 aa in size on the surface of the T7 capsid. Target sequences are fused to the C-terminus of the 10B capsid protein near amino acid 348. T7Select10 phage are grown on a complementing host (BLT5403) that supplies large amounts of the 10A capsid protein from a plasmid. Capsids thus contain mostly 10A protein, along with 5–15 copies of 10B fusion protein per virion. The linear genome of T7Select10-3b is 36,249 bp in size. Unique restriction sites are shown on the vector map (TB243).


      The T7Select System is sold for research use only. Any commercial use of this Product, including drug discovery or development of commercial products, requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
      Catalogue Number70548
      Brand Family Novagen®
      References
      Product Information
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      T7Select™10-3b DNA SDS

      Title

      Safety Data Sheet (SDS) 

      T7Select™10-3b DNA Certificates of Analysis

      TitleLot Number
      70548

      Citations

      Title
    • Eun-Min Cho, et al. (2007) Phage display cDNA cloning and expression analysis of hydrophobins from the entomopathogenic fungus Beauveria (Cordyceps) bassiana. Microbiology 153, 3438-3447.
    • Inge Roman, et al. (2006) Hunting interactomes of a membrane protein: obtaining the largest set of VDAC-interacting protein epitopes. Molecular and Cellular Proteomics 5, 1667-1680.
    • Z.J. Li, et al. (2003) Screening and identification of proteins interacting with p38 MAP kinase via T7 phage-display screening system. Di Yi Jun Yi Da Xue Xue Bao 23, 1131-1133.
    • Alexi Kurakin and Dale Bresden. (2002) Target-assisted iterative screening reveals novel interactors for PSD95, Nedd4, Src, Ab1 and Crk proteins. Journal of Biomolecular Structure and Dynamics 19,.
    • Kimmo Porkka, et al. (2002) A fragment of the HMGN2 protein homes to the nuclei of tumor cells and tumor endothelial cells in vivo. Procedings of the National Academy of Science 99, 7444-7449.
    • M.P. Burgoon, et al. (2001) Antigen discovery in chronic human inflammatory central nervous disease: panning phage-display antigen libraries identifies the targets of central nervous system-derived IgG in subacute sclerosing panencephalitis. Journal of Immunology 167, 6009-6014.
    • Mona H. Hansen, Bjorn Ostenstad and Mouldy Sioud. (2001) Identification of immunogenic antigens using a phage-displayed cDNA library from an invasive ductal breast carcinoma tumor. International Journal of Oncology 19, 1303-1309.
    • Paul P. Sche, et al. (1999) Display cloning: functional identification of natural product receptors using cDNA-phage dispaly. Chemistry and Biology 6, 707-716.
    • User Protocols

      Title
      TB178 T7Select® System Manual

      Vector Map

      Title
      TB243 T7Select® 10-3b Vector Map

      Vector Sequence

      Title
      T7Select® 10-3b Vector Sequence

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      Categories

      Life Science Research > Genomic Analysis > DNA Preparation & Cloning > Cloning > Cloning Vectors
      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors