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2140 Total Protein Extraction Kit

Total Protein Extraction Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Protein Extraction
2140
1 kit  
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      Overview

      Replacement Information
      Description
      Catalogue Number2140
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionTotal Protein Extraction Kit
      OverviewTotal protein extraction kit is an excellent tool for the initial purification and preparation of proteins from any tissues and cells. Total protein isolated by this kit is native and can be used for downstream applications including SDS-PAGE, Western blotting, gel mobility shift, protein assays and other procedures. The kit is easy to use: no scraping, no freeze-thaw cycles, and no sonication.

      This kit contains enough reagents for the isolation of protein from 5 grams of tissue or cultured cells.
      Materials Required but Not Delivered· Tissue Homogenizer

      · Centrifuge and Microcentrifuge

      · Rolling facility
      References
      Product Information
      Components
      • TM Buffer 13 mL (Stored at 2-8°C)
      • 50X PI Buffer 260 μL (Stored at -20°C)
      Applications
      Key Applications
      • Protein Extraction
      Application Notes· Isolate protein from large samples

      · Isolate protein either from tissues or from cultured cells

      · Protein is intact, and some proteins purified by this kit could be over 200 kd in mass

      The isolated protein can be used for Western Blot, DNA-protein interaction, enzymatic activity analysis, protein-protein interaction, and immunoprecipitation and tissue specific expression identification.

      PROTOCOL:

      Important:

      Aliquot 50X PI on ice and store at -20°C.

      Always make fresh working solutions before the isolation of proteins.

      1. Dilute 50X PI solution to 1X PI in TM buffer keeping the solution on ice.

      2. Weigh certain tissues and chop them into small pieces.

      3. Keep the tissues on dry ice.

      4. Add 1X PI to the tissue at 2.5 mL per gram of tissue or per 25 million cells, and put in ice for 5 minutes.

      5. Homogenize the tissue or cells for 20 seconds and then put on dry ice for 15 seconds.

      6. Homogenize the tissue or cells for the second 20 seconds. (A third 20 seconds homogenization may be required if the tissue or cells are not well homogenized.)

      7. Rotate the homogenized tissue or cells at 4°C for 20 minutes.

      8. Centrifuge at 11,000 rpm at 4°C for 20 minutes.

      9. Collect the supernatant.

      10. Determine the concentration of the total protein.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore the TM Buffer at 2-8°C for up to 12 months after date of receipt. Store the 50X PI Buffer at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      2140 04053252004391

      Documentation

      Total Protein Extraction Kit SDS

      Title

      Safety Data Sheet (SDS) 

      Total Protein Extraction Kit Certificates of Analysis

      TitleLot Number
      TOTAL PROTEIN EXTRACTION KIT VP1809143
      TOTAL PROTEIN EXTRACTION KIT VP1803160
      TOTAL PROTEIN EXTRACTION KIT 2856132
      TOTAL PROTEIN EXTRACTION KIT 2944808
      TOTAL PROTEIN EXTRACTION KIT 2925324
      TOTAL PROTEIN EXTRACTION KIT 2475625
      TOTAL PROTEIN EXTRACTION KIT 2901861
      TOTAL PROTEIN EXTRACTION KIT - 2135094 2135094
      TOTAL PROTEIN EXTRACTION KIT - 2390373 2390373
      TOTAL PROTEIN EXTRACTION KIT - 1975920 1975920
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