71186 YeastBuster™ Protein Extraction Reagent

71186
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71186-3CN
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      Plastic ampoule 100 ml
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      Description
      OverviewYeastBuster™ Protein Extraction Reagent is formulated for a fast, efficient and gentle extraction of active proteins from yeast cells. The reagent avoids harsh conditions of vigorous mechanical or chemical treatment that often result in heat degradation of target proteins. The proprietary formulation utilizes a mix of mild detergent, protein stabilization buffer, and tris(hydroxypropyl) phosphine (THP) reducing agent (THP concentrate provided separately). This powerful combination eliminates the inconsistencies associated with tedious mechanical disruption of yeast cells with glass bead abrasives, ultrasonication and pressure disruption, or enzymatic digestion with β-1,3-glucanase lytic enzymes. The reagent has been tested with Saccharomyces cerevisiae, Pichia pastoris, P. stipidis, and Schizosaccharomyces pombe strains and with plant cells.

      In practice, cells are harvested by centrifugation and suspended in YeastBuster. Following a brief incubation, insoluble cell debris is removed by centrifugation, and the clarified extract is ready to use. In addition to greater total protein yields in crude extracts and recovery of enzymatically active protein, the extracts are fully compatible with GST•Bind™ and Ni-NTA His•Bind® immobilized metal affinity chromatography (IMAC) purification methods. The reagent is available in 100- and 500-ml sizes.








      Catalogue Number71186
      Brand Family Novagen®
      Features and benefits
      • Gentle, rapid, efficient extraction of proteins from yeast cells
      • Eliminates the inconsistencies associated with abrasive grinding, ultrasonication, and pressure disruption of yeast cells
      • Higher yield of total and enzymatically active proteins
      • Fully compatible with Ni-NTA His•Bind® and GST•Bind™ affinity purification methods
      References
      Product Information
      Components
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage Multiple storage requirements
      Do not freeze Ok to freeze
      Packaging Information
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      Supplemental Information
      Specifications

      Documentation

      YeastBuster™ Protein Extraction Reagent SDS

      Title

      Safety Data Sheet (SDS) 

      YeastBuster™ Protein Extraction Reagent Certificates of Analysis

      TitleLot Number
      71186

      Brochure

      Title
      Protein Purification and Detection Tools
      The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression
      Western Blotting Tools

      Technical Info

      Title
      TB316 YeastBuster Protein Extraction Reagent

      Citations

      Title
    • Jennifer A. Gibbons, et al. (2007) Expression of human protein phosphatase-1 in Saccharomyces cerevisiae highlights the role of phosphatase isoforms in regulating eukaryotic functions. Journal of Biological Chemistry 282, 21838-21847.
    • Alyssa Dilla, et al. (2004) The Arabidopsis F-Box protein SLEEPY1 targets gibberellin signaling repressors for gibberellin-induced degradation. The Plant Cell 16, 1392-1405.
    • User Protocols

      Title
      TB316 YeastBuster™ Extraction Reagent