70609 pETBlue™-2 DNA (uncut) - Novagen

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      Plastic ampoule 20 μg
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      OverviewThe pETBlue™-2 vector is designed to identify recombinants by traditional blue/white screening while also allowing T7lac promoter based expression of target genes. Screening is independent of expression because the T7lac expression promoter is in an opposed orientation relative to the E. coli promoter that mediates blue/white screening. pETBlue-2 defines the open reading frame and inserts must be cloned in-frame if expression is desired. The vector features an expanded multiple cloning site (MCS) and optional C-terminal HSV•Tag® and His•Tag® sequences. The sequence is numbered from the first base of the T7 promoter sequence.

      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
      Catalogue Number70609
      Brand Family Novagen®
      Product Information
      Quality LevelMQ100
      Biological Information
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information


      pETBlue™-2 DNA (uncut) - Novagen SDS


      Safety Data Sheet (SDS) 

      pETBlue™-2 DNA (uncut) - Novagen Certificates of Analysis

      TitleLot Number


    • Yoshitaka Sato, et al. (2006) Sumoylation of CCAAT/enhancer-binding protein alpha and its functional roles in hepatocyte differentiation. Journal of Biological Chemistry 281, 21629-21639.
    • Michael D. Urbaniak, et al. (2005) The N-Acetyl-D-glucosaminylphosphatidylinositol De-N-acetylase of glycosylphosphatidylinositol biosynthesis Is a zinc metalloenzyme. Journal of Biological Chemistry 280, 22831-22838.
    • Cynthia L. Richard, Animesh Tandon and Robert G. Kranz. (2004) Rhodobacter capsulatus nifA1 promoter: high-GC -10 regions in High-GC bacteria and the basis for their transcription. Journal of Bacteriology 186, 740-749.
    • Céline Pujol and James B. Bliska. (2003) The ability to replicate in macrophages Is conserved between Yersinia pestis and Yersinia pseudotuberculosis. Infection and Immunity 71, 5892-5899.
    • Xianghong Li and A-Lien Lu. (2001) Molecular cloning and functional analysis of the mutY homolog of Deinococcus radiodurans. Journal of Bacteriology 183, 6151-6158.
    • Sheng Yuan, Yajun Wu and Daniel J. Cosgrove. (2001) A fungal endoglucanase with plant cell wallextension activity. Plant Physiology 127, 324-333.
    • User Protocols

      TB249 pETBlue™ System Manual

      Vector Map

      TB259VM pETBlue™-2 Vector Map