Cell Migration Assay Chart |
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| Description | Pore Size | Plate Format | ECM Coating | Detection | # Tests | Cat. No. |
|---|---|---|---|---|---|---|
| Chemotaxis Cell Migration Assays | 8 µm | 24-well | None | Colorimetric | 24 | ECM508 |
| 24-well | Fluorometric | 24 | ECM509 | |||
| 96-well | Fluorometric | 96 | ECM510 | |||
| 5 µm | 24-well | Colorimetric | 24 | ECM506 | ||
| 24-well | Fluorometric | 24 | ECM507 | |||
| 96-well | Fluorometric | 96 | ECM512 | |||
| 3µm | 24-well | Colorimetric | 24 | ECM504 | ||
| 24-well | Fluorometric | 24 | ECM505 | |||
| 96-well | Fluorometric | 96 | ECM515 | |||
| Haptotaxis Cell Migration Assays | 8 µm | 24-well | Fibronectin | Colorimetric | 24 | ECM580 |
| 24-well | Vitronectin | Fluorometric | 24 | ECM581 | ||
| 24-well | Collagen I | Fluorometric | 24 | ECM582 | ||
| 5 µm | 24-well | Laminin vials | Colorimetric | 24 | ECM220 | |
| 24-well | Fluorometric | 24 | ECM221 | |||
| Millicell® µ-Migration Assay Kit | NA | 4 slides of 3 wells | NA | 12 | MMA205 | |
Migration & Invasion Assay Chart |
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What pore size should I select?
Pore size determination depends entirely on your cell type. A quickliterature search will enable you to decide the best pore size for the particular cells you are using. The following chart illustrates pore size choice for cell lines used in our laboratory and by some of our customers for these assays.| Cell Name | Cell Type | Pore Sizes Typically Used | Assays Typically Performed |
|---|---|---|---|
| MDA-MB-231 | Invasive breast cancer cell line (human) | 5 or 8 µm | 5 or 8 µm used in chemotaxis or invasion assay. |
| MCF7 | Non-invasive breast cancer cell line (human) | 5 or 8 µm | 5 or 8 µm used in chemotaxis or invasion assay. |
| HT1080 | Invasive fibrosarcoma cell line (human) | 5 or 8 µm | 5 or 8 µm used in chemotaxis or invasion assay. |
| NIH3T3 | Non-invasive fibroblast cell line (mouse) | 5 or 8 µm | 5 or 8 µm used in chemotaxis or invasion assay. |
| HUVEC (Human vein umbilical vein endothelial cells) | Endothelial cells | 3 or 5 or 8 µm | 3 or 5 or 8 µm in chemotaxis, invasion, angiogenesis or transendothelial migration assays. |
| HMVEC/HMEC (Human dermal microvascular) | Endothelial cells | 5 or 8 µm | 5 or 8 µm in chemotaxis, invasion, angiogenesis or transendothelial migration assays. |
| PMN | Polymorphonuclear neutrophils | 1 or 3 µm | 1 or 3 µm in chemotaxis assays. |
| Primary Stromal cells | 8 µm | No information available. | |
| Epithelial cells | 3 or 5 µm | No information available. | |
| Human coronary artery smooth muscle cells | 5 µm | No information available. | |
| Hepatic stellate cells | Myofibroblast | 5 µm | No information available. |