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Merck

860336

3,3′,5,5′-Tetramethylbenzidine

≥99%

Synonym(s):

BM blue, Sure Blue TMB, TMB, TMB Blotting Plus, TMB substrate

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About This Item

Linear Formula:
[-C6H2(CH3)2-4-NH2]2
CAS Number:
54827-17-7
Molecular Weight:
240.34
UNSPSC Code:
12352100
NACRES:
NA.22
PubChem Substance ID:
24888505
EC Number:
259-364-6
Beilstein/REAXYS Number:
2808541
MDL number:
MFCD00007748

Product Name

3,3′,5,5′-Tetramethylbenzidine, ≥99%

InChI

1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

InChI key

UAIUNKRWKOVEES-UHFFFAOYSA-N

SMILES string

Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

assay

≥99%

form

powder

mp

168-171 °C (lit.)

storage temp.

2-8°C

Quality Level

200

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Application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

General description

3,3′,5,5′-Tetramethylbenzidine is a peroxidase sensitive dye. It is a better alternative to syringaldazine for the determination of chlorine in water due to its product stability and no interference from calcium and magnesium ions.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
WXBF7859V
WXBF5835V
WXBF4985V
WXBF2613V
WXBF2433V

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Colorimetric method for determination of chlorine with 3, 3?, 5, 5?-tetramethylbenzidine.
Serrat F B
Talanta, 41(12), 2091-2094 (1994)
Iron oxide nanoparticles: Hidden talent
Perez J M
Nature Nanotechnology, 2(9), 535-535 (2007)
Y Jung et al.
Mucosal immunology, 8(4), 930-942 (2015-01-08)
Eosinophils are multifunctional leukocytes that reside in the gastrointestinal (GI) lamina propria, where their basal function remains largely unexplored. In this study, by examining mice with a selective deficiency of systemic eosinophils (by lineage ablation) or GI eosinophils (eotaxin-1/2 double
Jan Pencik et al.
Nature communications, 6, 7736-7736 (2015-07-23)
Prostate cancer (PCa) is the most prevalent cancer in men. Hyperactive STAT3 is thought to be oncogenic in PCa. However, targeting of the IL-6/STAT3 axis in PCa patients has failed to provide therapeutic benefit. Here we show that genetic inactivation
Jiabin Yan et al.
Viruses, 7(3), 1429-1453 (2015-03-26)
Signaling through the Fas/Apo-1/CD95 death receptor is known to affect virus-specific cell-mediated immune (CMI) responses. We tested whether modulating the Fas-apoptotic pathway can enhance immune responses to DNA vaccination or lymphocytic choriomeningitis virus (LCMV) infection. Mice were electroporated with plasmids
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