Key Documents
05-1149
Anti-ADP-Ribosylation Factor 6 Antibody, clone 6ARF01
clone 6ARF01, from mouse
Synonym(s):
ADP-ribosylation factor 6
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
General description
ADP-Ribosylation Factor 6 (ARF6) is a GTP-binding protein that functions as a regulator of cytoskeleton remodeling and endocytic recycling. ARF6 is an allosteric activator of cholera toxin catalytic subunit, an ADP-ribosyltransferase. It may also be involved in the modulation of vesicle budding and uncoating with the Golgi apparatus.
Approx. 19-20 kDa
Immunogen
Recombinant human ARF6 protein.
Application
Anti-ADP-Ribosylation Factor 6 Antibody, clone 6ARF01 is a Mouse Monoclonal Antibody for detection of ADP-Ribosylation Factor 6 also known as ADP-ribosylation factor 6 & has been validated in WB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
Biochem/physiol Actions
Recognizes ADP-ribosylation factor (ARF) 6.
Physical form
Format: Purified
Protein G Chromatography
Purified monoclonal IgG ascites in 10 mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
Preparation Note
Maintain refrigerated at 2-8ºC in undiluted aliquots for up to 12 months from date of receipt.
Analysis Note
Control
NIH/3T3 Cell Lysate
NIH/3T3 Cell Lysate
Evaluated in Western Blot on NIH/3T3 cell lysates.
Western Blot Analysis: 1:500 dilution of this antibody detected ARF6 in 10 µg of NIH/3T3 cell lysate.
Western Blot Analysis: 1:500 dilution of this antibody detected ARF6 in 10 µg of NIH/3T3 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Chadwick T Davis et al.
Journal of immunology (Baltimore, Md. : 1950), 192(12), 6045-6052 (2014-05-20)
The vascular endothelium responds to infection by destabilizing endothelial cell-cell junctions to allow fluid and cells to pass into peripheral tissues, facilitating clearance of infection and tissue repair. During sepsis, endotoxin and other proinflammatory molecules induce excessive vascular leak, which
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