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Merck

05-1307-AF488

Anti-Ubiquitin, Lys48-Specific, clone Apu2, Alexa Fluor™ 488 conjugate

clone Apu2, from rabbit, ALEXA FLUOR™ 488

Synonym(s):

Polyubiquitin-B, Ubiquitin, Lys48-Specific, Ubiquitin

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

conjugate

ALEXA FLUOR™ 488

antibody form

purified antibody

antibody product type

primary antibodies

clone

Apu2, monoclonal

species reactivity

rat, mouse, human

species reactivity (predicted by homology)

rabbit (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable

isotype

IgG

NCBI accession no.

NP_001268645.1

UniProt accession no.

P0CG47

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... UBB(7314)
mouse ... Ubg(107729)

General description

Polyubiquitin-B (UniProt P0CG47) is encoded by the UBB gene (Gene ID 7314) in human. Ubiqutin (Ub) is initially produced as a 229-a.a. precursor protein whose C-terminus Cys229 is post-translationally removed, followed by cleavages between a.a. 76 and 77, as well as between a.a. 152 and 153 to yield three idential 76-amino acid Ub molecules. Ub is involved in one of the most common post-translational modifications (PTMs) of cellular proteins, where Ub is linked covalently via its carboxyl terminus to usually Lys residues in target proteins. A given target Lys residue can be linked to one single Ub molecule (monoubiquitylated) or to a chain of Ub molecules (polyubiquitylated). In a polyubiquitin chain, ubiquitin molecules can be linked through one of the seven ubiquitin Lys residues (K6, K11, K27, K29, K33, K48, and K63) or through the Ub N-terminus Met1 residue (which generates linear chains). An Ub chain with a single linkage type is referred to as homotypic, whereas an heterotypic chain contains mixed linkages within the same chain, which can lead to branched/forked (as a result of one Ub linked to two or more Ubs) or non-branched Ub chains. Lys48-linked chains mark proteins for proteasomal degradation, while Lys63-linked chains regulate the activity/function of cellular proteins.
~17 kDa observed

Immunogen

Epitope: Lys48 ubiquitination site
Immunogen used was human di-ubiquitin linked at Lys48.

Application

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Ubiquitin in A431 and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 05-1307) is shown to be suitable also for cell funtion assay, flow cytometry, immunocytochemistry, immunohistochemistry, immunoprecipitation, Western blotting applications.
Research Category
Signaling
Research Sub Category
Developmental Signaling
This Anti-Ubiquitin, Lys48-Specific, clone Apu2, Alexa Fluor™ 488 conjugate is validated for use in Immunocytochemistry for the detection of Ubiquitin.

Biochem/physiol Actions

Based on sequence homology, broad species cross-reactivity is expected.
Recognizes ubiquitin linked at Lys48. Does not recognize ubiquitin linked at Lys63 or free ubiquitin.

Physical form

Protein A purified
Purified rabbit antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Ubiquitin in HeLa cells.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Samuel S Pappas et al.
Human molecular genetics, 27(3), 407-420 (2017-12-01)
A critical challenge to deciphering the pathophysiology of neurodevelopmental disease is identifying which of the myriad abnormalities that emerge during CNS maturation persist to contribute to long-term brain dysfunction. Childhood-onset dystonia caused by a loss-of-function mutation in the AAA+ protein torsinA
Christopher S Morrow et al.
Cell stem cell, 26(4), 558-568 (2020-02-29)
Maintaining a healthy proteome throughout life is critical for proper somatic stem cell function, but the complexities of the stem cell response to increases in damaged or aggregated proteins remain unclear. Here we demonstrate that adult neural stem cells (NSCs)
Xin Wang et al.
Chemical biology & drug design, 86(5), 1036-1048 (2015-04-10)
The ubiquitin-proteasome system (UPS) is increasingly recognized as a therapeutic target for the development of anticancer therapies. The success of the 20S proteasome core particle (20S CP) inhibitor bortezomib in the clinical management of multiple myeloma has raised the possibility

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