Anti-phospho-Histone H2B (Ser14) Antibody

15 kDa

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various posttranslational modifications, including acetylation, phosphorylation, methylation and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression.

Broad species cross-reactivity expected based on sequence homology.

Recognizes phospho-Histone H2B (Ser14), Mr 15 kDa.

Epitope: Ser14

KLH-conjugated, synthetic peptide corresponding to amino acids 6-16 of human histone H2B (C-SAPAPKKG[pS]KK).

Use Anti-phospho-Histone H2B (Ser14) Antibody (Rabbit Polyclonal Antibody) validated in ELISA, ICC, IHC, WB to detect phospho-Histone H2B (Ser14) also known as H2BS14P, Histone H2B (phospho S14).

Rabbit antiserum polyclonal IgG in buffer containing 0.05% sodium azide and 30% glycerol. Liquid at -20°C.

Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Control
Acid extracted HL-60 cells treated with VP16

Routinely evaluated by western blot on acid extracted HL-60 cells treated with VP16.

Western Blot Analysis:
1:1,000-1:10,000 dilutions of this lot detected phospho-Histone H2B in acid extracted HL-60 cells treated with 20μg/mL VP16 for 18 hours. A 1:250-1:2,000 dilution of a previous lot also detected phospho-Histone H2B acid extracted proteins from colcemid-arrested HeLa cells.

Note:
The optimal antibody dilution of this antibody must be empirically determined. Use of this antibody at dilutions which are too low, will result in non-specific detection of Histone H2B and other unknown proteins in western blots of acid extracts from untreated cells.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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