07-191
Anti-phospho-Histone H2B (Ser14) Antibody
serum, Upstate®
Synonym(s):
H2BS14P, Histone H2B (phospho S14), H2B type 12, histone 3, H2bb, histone H2B, v
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
General description
15 kDa
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various posttranslational modifications, including acetylation, phosphorylation, methylation and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression.
Immunogen
Epitope: Ser14
KLH-conjugated, synthetic peptide corresponding to amino acids 6-16 of human histone H2B (C-SAPAPKKG[pS]KK).
Application
Use Anti-phospho-Histone H2B (Ser14) Antibody (Rabbit Polyclonal Antibody) validated in ELISA, ICC, IHC, WB to detect phospho-Histone H2B (Ser14) also known as H2BS14P, Histone H2B (phospho S14).
Biochem/physiol Actions
Broad species cross-reactivity expected based on sequence homology.
Recognizes phospho-Histone H2B (Ser14), Mr 15 kDa.
Physical form
Rabbit antiserum polyclonal IgG in buffer containing 0.05% sodium azide and 30% glycerol. Liquid at -20°C.
Preparation Note
Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Control
Acid extracted HL-60 cells treated with VP16
Acid extracted HL-60 cells treated with VP16
Routinely evaluated by western blot on acid extracted HL-60 cells treated with VP16.
Western Blot Analysis:
1:1,000-1:10,000 dilutions of this lot detected phospho-Histone H2B in acid extracted HL-60 cells treated with 20μg/mL VP16 for 18 hours. A 1:250-1:2,000 dilution of a previous lot also detected phospho-Histone H2B acid extracted proteins from colcemid-arrested HeLa cells.
Note:
The optimal antibody dilution of this antibody must be empirically determined. Use of this antibody at dilutions which are too low, will result in non-specific detection of Histone H2B and other unknown proteins in western blots of acid extracts from untreated cells.
Western Blot Analysis:
1:1,000-1:10,000 dilutions of this lot detected phospho-Histone H2B in acid extracted HL-60 cells treated with 20μg/mL VP16 for 18 hours. A 1:250-1:2,000 dilution of a previous lot also detected phospho-Histone H2B acid extracted proteins from colcemid-arrested HeLa cells.
Note:
The optimal antibody dilution of this antibody must be empirically determined. Use of this antibody at dilutions which are too low, will result in non-specific detection of Histone H2B and other unknown proteins in western blots of acid extracts from untreated cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Histone deimination as a response to inflammatory stimuli in neutrophils.
Indira Neeli,Salar N Khan,Marko Radic
Journal of immunology (Baltimore, Md. : 1950) (1950)
A semisynthetic strategy to generate phosphorylated and acetylated histone H2B.
Chiang, KP; Jensen, MS; McGinty, RK; Muir, TW
Chembiochem null
Histone modifications associated with somatic hypermutation.
Odegard, VH; Kim, ST; Anderson, SM; Shlomchik, MJ; Schatz, DG
Immunity null
Mi-2/NuRD complex function is required for normal S phase progression and assembly of pericentric heterochromatin.
Sims, JK; Wade, PA
Molecular Biology of the Cell null
Dafni Eleftheria Pefani et al.
The EMBO journal, 37(15) (2018-05-24)
The heavily transcribed rDNA repeats that give rise to the ribosomal RNA are clustered in a unique chromatin structure, the nucleolus. Due to its highly repetitive nature and transcriptional activity, the nucleolus is considered a hotspot of genomic instability. Breaks
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