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119101

Sigma-Aldrich

Anti-H+,K+-ATPase, α-Subunit (1019-1034) Rabbit pAb

liquid, Calbiochem®

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About This Item

UNSPSC Code:
12352203

biological source

rabbit

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

rat, human, canine, mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−70°C

General description

This product has been discontinued.



Recognizes the α-subunit of H+,K+-ATPase.

  • Antibody Target Gene Symbol: ATP4A
  • Target Synonym: ATP6A, Atpase, h+/k+ transporting, alpha polypeptide, Gastric H+/K+ ATPase Alpha, H+/K+ ATPASE, HKATPC, RATHKATPC
  • Entrez Gene Name: ATPase, H+/K+ exchanging, alpha polypeptide
  • Hu Entrez ID: 495 (Related Antibodies: 119102)
  • Mu Entrez ID: 11944
  • Rat Entrez ID: 24216

    • Anti-H+,K+-ATPase, α-Subunit (1019-1034), rabbit polyclonal, recognizes the α-subunit of H+,K+-ATPase. It is validated for use in ELISA, WB, ICC, and IHC (paraffin sections).
    Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the α-subunit of H+,K+-ATPase.

    Immunogen

    Porcine
    a synthetic peptide (GVRCCPGSWWDQGLYY) corresponding to amino acids 1019-1034 of porcine H+, K+-ATPase α-subunit

    Application

    ELISA (see comments)

    Immunoblotting (1:1000)

    Immunocytochemistry (see comments)

    Paraffin Sections (see comments)

    Physical form

    Undiluted serum.

    Preparation Note

    Following initial thaw, aliquot and freeze (-70°C). Recommended dilution buffer is Tris buffered saline with 1% BSA, pH 7.5.

    Other Notes

    Exhibits some cross-reactivity with Na+, K+-ATPase at working dilution for H+,K+-ATPase. This antibody is also reported to work for ELISA, immunocytochemistry, and paraffin sections. Variables associated with assay conditions will dictate the proper working dilution.
    Smolka, A. 1992. Biophys. Biochem. Acta 1108, 75.

    Legal Information

    CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

    Disclaimer

    Toxicity: Standard Handling (A)

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    Storage Class Code

    10-13 - German Storage Class 10 to 13

    Certificates of Analysis (COA)

    Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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