ChIPAb+ CHD1

All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ CHD1 validated antibody and primer set includes a CHD1 antibody, a Normal Rabbit IgG, and positive control primers which amplify 114 bp of human PSMC6 (proteasome 26S subunit 6) The CHD1 antibody and negative control IgG are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of CHD1 associated chromatin.

Recognizes CHD1 in regions between a.a. 1660-1710.

Chromatin Immunoprecipitation (ChIP) Western Blotting

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from K562 cells (~4e6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 0.7 µg of either Normal Rabbit IgG (Part No. PP64B), or 0.7 µg Anti-CHD1 (Part No. CS216151) and the Magna ChIP HiSens (Cat. # 17-10460). Successful immunoprecipitation of CHD1 associated DNA fragments was verified by qPCR using ChIP Primers, PSMC6 (Part No. CS216152) as a positive locus, and ChIP Primers, SNRPN (Part No. CS207347) as a negative locus.
Please refer to the Magna ChIP HiSens (Cat. # 17-10460) or EZ-MagnaChIP HiSens(Cat. # 17-10461) protocol for experimental details.
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from K562 cells (1e6, 4e6 and 1e7 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 0.7 µg of either Normal Rabbit IgG (Part No. PP64B), or 0.7 µg Anti-CHD1 (Part No. CS216151) and the Magna ChIP HiSens Kit (Cat. # 17-10460). Successful immunoprecipitation of CHD1 associated DNA fragments was verified by qPCR using ChIP Primers, PSMC6 (Part No. CS216152).
Please refer to the MagnaChIP HiSens (Cat. # 17-10460) or EZ-MagnaChIP HiSens (Cat. # 17-10461) protocol for experimental details.
Western Blotting Analysis:
0.04 µg/mL of this antibody detected CHD1 on 5, 15 and 50 µg of HeLa and 293T whole cell lysate.

This ChIPAb+ Validated Antibody & Primer Set conveniently includes the antibody, matched IgG negative control antibody & set control PCR primers that detect a known positive locus.

Recombinant protein corresponding to human CHD1 (a.a. 1660-1710).

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Anti-CHD1 (Rabbit Polyclonal), Part No. CS216151. One vial containing 7.0 µg purified rabbit polyclonal in 50 µL buffer of Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide, before the addition of 30% glycerol (0.14 mg/mL final). Store at -20°C.Normal Rabbit IgG, Part No. PP64B. One vial containing 125 μg of purified Rabbit IgG in 125 μL storage buffer containing 0.05% sodium azide. Store at -20°C.ChIP Primers, PSMC6. Part No. CS216152. One vial containing 75 μL of 5 μM of each primer specific for human proteasome 26S subunit 6 (chr14:53174161+53174274 , hg19 build). Primer location selection based on ENCODE browser data at UCSC, see also reference 1. Store at -20°C. FOR: TCC GGC CCT GAG CTT GTREV: CCT CCT CTA CTT CTT TTT CAT TTT CAC

Chromodomain-helicase-DNA-binding protein 1 (CHD1) is a multidomain chromatin-remodelling enzyme, which belongs to the SNF2/RAD54 helicase family. It contains an SWI/SNF-like helicase/ATPase domain, a DNA-binding domain, and two chromodomains. CHD1 is multifunctional protein, which has an important role in gene regulation. It interacts with various modified histones, such as H3K4Me2 and H3K4Me3, and maintains the open chromatin structure required for transcription. It also interacts with, or plays a role in the assembly of, several transcription-related complexes, including the histone acetylation complex SAGA, and the FACT and PAF complexes. Previous studies have suggested that CHD1 maintains the pluripotent state of embryonic stem cells, and is required for the reprogramming of somatic cells to the pluripotent state. CHD1 may play a role in the development of prostate cancer.

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from K562 cells fixed for 10 min (4e6 cell equivalents per IP) was subjected to chromatin immunoprecipitation using 0.7 µg (0.7 µL) of either Normal Rabbit IgG (Part No. PP64B), or 0.7 µg (5 µL) Anti-CHD1 (Part No. CS216151) and the Magna ChIP HiSens Kit (Cat. # 17-10460). Successful immunoprecipitation of CHD1 associated DNA fragments was verified by qPCR using ChIP Primers, PSMC6 (Part No. CS216152).
Please refer to the MagnaChIP HiSens (Cat. # 17-10460) or EZ-MagnaChIP HiSens (Cat. # 17-10461) protocol for experimental details.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.