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176900

Sigma-Aldrich

Anthrax Lethal Factor, Recombinant, Bacillus anthracis, B. anthracis - Calbiochem

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About This Item

UNSPSC Code:
12352202

form

lyophilized

Quality Level

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

shipped in

ambient

storage temp.

2-8°C

General description

This product has been discontinued.



Recombinant, Bacillus anthracis anthrax lethal factor expressed in a specialized strain of B. anthracis. One of the three protein components of Anthrax toxin, lethal factor (LF) is a highly specific protease that cleaves members of the mitogen-activated protein kinase kinase (MAPKK) family. LF is comprised of four domains. Domain I binds the protective antigen to enter the target cell; domains II, III, and IV create a long groove to hold and cleave the MAPKK proteins.

Recombinant, Bacillus anthracis anthrax lethal factor expressed in a special strain of B. anthracis. One of three protein components of anthrax toxin produced by the pathogenic bacterium, Bacillus anthracis. Highly specific protease component that cleaves members of the mitogen-activated protein kinase kinase (MAPKK) family. LF is comprised of four domains: domain I that binds to protective antigen (necessary for entry into the cellular target) and domains II, III, and IV which form a long groove to hold and cleave the target proteins. Requires passage through an acidic vesicle prior to translocation to the cytoplasm of the cell. Cleavage of target proteins leads to the inhibition of multiple signaling pathways and cell death.

Biochem/physiol Actions

Primary Target
MAPKK

Packaging

yes

Physical form

Lyophilized from 50 mM NaCl, 5 mM HEPES, pH 7.5.

Preparation Note

Following reconstitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 6 months at -20°C.
Please refer to the vial label for lot-specific reconstitution volume.

Analysis Note

Single major band by SDS-PAGE

Other Notes

Brossier, F., and Mock, M. 2001. Toxicon39, 1747.
Friedlander, A.M. 2001. Nature414, 160.
Gupta, P., et al. 2001. Biochem. Biophys. Res. Commun.284, 568.
Pannifer, A.D., et al. 2001. Nature414, 160.
Pugsley, A.P. 1996. Proc. Natl. Acad. Sci. USA93, 8155.
Leppla, S.H. 1991. Methods Enzymol.195, 153.
One unit is defined as the amount of enzyme required to catalyze the release of 1.0 pmole of cleaved MAPKKide substrate per min at 37°C, in 20 mM HEPES, pH 8.2.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class Code

10-13 - German Storage Class 10 to 13


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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