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209550

Sigma-Aldrich

Anti-Cannabinoid Receptor CB₁ (1-77) Rabbit pAb

liquid, Calbiochem®

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About This Item

UNSPSC Code:
12352203

biological source

rabbit

Quality Level

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

rat, gerbil, mouse, salamander, chicken, monkey, fish, human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

General description

Anti-Cannabinoid Receptor CB₁ (1-77), rabbit polyclonal, recognizes the ~60 kDa CB1 receptor in wide variety of vertebrates. It is validated for use in immunoblotting and with paraffin sections.
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~60 kDa CB1 receptor protein.
Recognizes the ~60 kDa CB1 receptor protein.

Immunogen

Rat
a recombinant protein consisting of the first 77 amino acids of rat CB₁ receptor

Application

Immunoblotting (1:1000)

Immunocytochemistry (1:100-1:1000)

Paraffin Sections (see comments)

Physical form

In PBS, 1 mg/ml BSA, 50% glycerol.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Hippocampal neurons

Other Notes

May also recognize weaker bands at ~23 kDa, ~72 kDa, and ~180 kDa in some tissues. Immunocytochemical staining of living or fixed cultured hippocampal neurons show intense, punctate staining along processes with little staining over cell bodies. Variables associated with assay conditions will dictate the optimal working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class Code

10-13 - German Storage Class 10 to 13


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Angelo Blasio et al.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, 38(12), 2498-2507 (2013-06-25)
The anti-obesity medication rimonabant, an antagonist of cannabinoid type-1 (CB(1)) receptor, was withdrawn from the market because of adverse psychiatric side effects, including a negative affective state. We investigated whether rimonabant precipitates a negative emotional state in rats withdrawn from
Francesca Guida et al.
Brain, behavior, and immunity, 85, 128-141 (2019-04-07)
Recent evidence points to the gut microbiota as a regulator of brain and behavior, although it remains to be determined if gut bacteria play a role in chronic pain. The endocannabinoid system is implicated in inflammation and chronic pain processing
Jean-François Bouchard et al.
Life sciences, 72(16), 1859-1870 (2003-02-15)
The aim of the present study was to assess the contribution of endogenous cannabinoids in the protective effect of ischemic preconditioning on the endothelial function in coronary arteries of the rat. Isolated rat hearts were exposed to a 30-min low
Talita H Ferreira-Vieira et al.
Hippocampus, 24(1), 79-88 (2013-10-12)
It is well known that physical exercise has positive effects on cognitive functions and hippocampal plasticity. However, the underlying mechanisms have remained to be further investigated. Here we investigated the hypothesis that the memory-enhancement promoted by physical exercise relies on
Rodrigo Del Rio et al.
Frontiers in synaptic neuroscience, 15, 1176864-1176864 (2023-05-30)
Cannabis exposure during gestation evokes significant molecular modifications to neurodevelopmental programs leading to neurophysiological and behavioral abnormalities in humans. The main neuronal receptor for Δ9-tetrahydrocannabinol (THC) is the type-1 cannabinoid receptor CB1R, one of the most abundant G-protein-coupled receptors in

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