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218709

Supelco

Casein Kinase II, α2, Active, Human, Recombinant, E. coli

Synonym(s):

CK2α2, CSNK2A2

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About This Item

UNSPSC Code:
12352202

Assay

≥90% (SDS-PAGE)

Quality Level

100

form

liquid

specific activity

≥841.819 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

dry ice

storage temp.

−70°C

General description

This product has been discontinued.



Recombinant, human casein kinase II, α2, (CKII, α2) fused to maltose binding protein (MBP) at the N-terminus and expressed in E. coli. CKII, α2 is a catalytic subunit of the serine/threonine protein kinase CKII. It is suitable for use in reactions where autophosphorylation of the regulatory CKII β-subunit is not desired. Shown to be extremely salt-sensitive with respect to its activity.

Recombinant, human casein kinase II, α2, (CKII, α2) fused to maltose binding protein (MBP) at the N-terminus and expressed in E. coli. CKII, α2 is a catalytic subunit of the serine/threonine protein kinase CKII. It is suitable for use in reactions where autophosphorylation of the regulatory CKII β-subunit is not desired. Shown to be extremely salt-sensitive with respect to its activity.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM Tris-HCl, 500 mM NaCl, 10 mM DTT, pH 8.5.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Antonelli, M., et al. 1997. Eur. J. Biochem.241, 272.
Xu, X., et al. 1997. Genomics48, 79.
Boldyreff, B., et al. 1994. Cell. Mol. Biol. Res.40, 391.
One unit is defined as the amount of enzyme required to transfer 1 pmol phosphate to RRRADDSDDDDD peptide substrate per min at 30°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Pictograms

Health hazard
GHS08

Signal Word

Warning

Hazard Statements

H373

Precautionary Statements

P260 - P314 - P501

Hazard Classifications

STOT RE 2 Oral

Target Organs

Thyroid

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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