Caspase Inhibitor Set IV - Calbiochem

A set of four ready-to-use cell-permeable, irreversible inhibitors of various caspase-family proteases and a negative control apoptosis inducer. Each vial contains 25 µl of a 10 mM solution in DMSO of Caspase-3 Inhibitor II, Z-DEVD-FMK (Cat. No. 264155); Caspase-8 Inhibitor II, Z-IETD-FMK (Cat. No. 218759); Caspase-9 Inhibitor I, Z-LEHD-FMK (Cat. No 218761); general caspase inhibitor, Z-VAD-FMK (Cat. No. 627610); the negative control Z-FA-FMK (Cat. No. 342000), and an aqueous solution (100 µl, 6.1 µg/µl) of an apoptosis inducer, Doxorubicin, Hydrochloride (Cat. No. 324380). Supplied with a data sheet.

Apoptosis is a normal process in development and morphogenesis. Many cells can be activated to undergo apoptosis following the interaction of selected ligands with cell surface receptors. Receptor-mediated apoptosis involves the activation of caspases (cysteine-containing aspartate-specific proteases). A distinctive feature of caspases is the requirement of an aspartic acid residue in the substrate P₁ position.



Caspase inhibitors act by binding to the active site of caspases and form either a reversible or an irreversible linkage. Caspase inhibitor design includes a peptide recognition sequence attached to a functional group such as an aldehyde (CHO), chloromethylketone (CMK), fluoromethylketone (FMK), or fluoroacyloxymethylketone (FAOM). Caspase inhibitors with a CHO group are reversible and those with a CMK, FMK, or FAOM group are irreversible and cell-permeable. FMK exhibits slightly less reactivity than CMK and therefore is more specific for the enzyme being inhibited.

Cell permeable: yes

Primary Target
pan-caspase

Product does not compete with ATP.

Reversible: no

Packaged under inert gas

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Multiple Toxicity Values, refer to MSDS (O)