234155
Collagenase, Type II, Clostridium histolyticum
Collagenase, Type II, Clostridium histolyticum, is a crude collagenase preparation that contains higher clostripain and tryptic activity than Type I.
Synonym(s):
Collagenase, Type II, Clostridium histolyticum
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About This Item
Quality Level
form
lyophilized
specific activity
≥200 collagenase units/mg dry wt
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated
solubility
aqueous buffer: soluble
foreign activity
Non-specific proteolytic ≥50 fluorescence units/mg
shipped in
ambient
storage temp.
2-8°C
General description
Crude collagenase preparation that contains higher clostripain and tryptic activity than Type I. Useful in tissue dissociation studies in which a combination of collagenase and high proteolytic activity is required.
Crude collagenase solid prepared to contain higher clostripain and tryptic activity than Type I. Useful in tissue dissociation studies in which a combination of collagenase and high proteolytic activity is required. Has an optimal pH of 7.0-8.0.
Preparation Note
Unstable in solution; reconstitute just prior to use.
Other Notes
Keller, S., and Mandl, I. 1963. Arch. Biochem. Biophys.101, 81.
One unit is defined as the amount of enzyme that will release 1.0 nmol Leu equivalent from collagen per min at 37°C, pH 7.2.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Harmful (C)
Storage Class Code
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
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