Key Documents
form
liquid
General description
Mixture of purified pertussis toxin-sensitive G-proteins from bovine brain. Suitable for functional studies of G-proteins. Contains the following heterotrimeric G-proteins: Goα (4 - 5 μM), Giα-1 (1 - 2 μM), Giα-2 (1 - 2 μM), and Giα-3 (<1 μM). The βγ-subunit complex associated with these α-subunits represents the predominant form found in bovine brain. Characterized by GTPγS binding activity and by immunoblotting using subunit-specific antisera.
Application
Suitable for GTPγS binding assay and Immunoblotting
Preparation Note
Following initial thaw, aliquot and freeze (-70°C)
Other Notes
Characterized by GTPγS binding and in immunoblot using subunit-specific antisera. Titration is recommended.
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P Goldsmith et al.
The Journal of biological chemistry, 262(30), 14683-14688 (1987-10-25)
Antisera AS/6 and 7, raised against a synthetic peptide KENLKDCGLF corresponding to the carboxyl-terminal decapeptide of transducin-alpha, react on immunoblots with purified transducin-alpha and with proteins of 40-41 kDa in all tissues tested. The latter represent one or more forms
Purification of recombinant Gi alpha and Go alpha proteins from Escherichia coli.
M E Linder et al.
Methods in enzymology, 195, 202-215 (1991-01-01)
Assay of G-protein beta gamma-subunit complex by catalytic support of ADP-ribosylation of Go alpha.
P J Casey et al.
Methods in enzymology, 195, 315-321 (1991-01-01)
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