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Key Documents

475702

Sigma-Aldrich

MEK1, GST-Fusion, Human, Recombinant, E. coli

Synonym(s):

MAP Kinase Kinase, ERK Kinase 1, Microtubule-associated protein 2 kinase

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About This Item

UNSPSC Code:
12352202

Assay

≥80% (SDS-PAGE)

Quality Level

100

form

liquid

specific activity

≥250,000 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−70°C

General description

This product has been discontinued.



Recombinant, human MEK1 fused to GST at the N-terminus and expressed in E. coli. MEK1 is a member of a dual-specificity protein kinase family that phosphorylates threonine and tyrosine residues on ERK 1 and 2.

Recombinant, human MEK1 fused to GST at the N-terminus and expressed in E. coli. MEK1 is a member of a dual-specificity protein kinase family that phosphorylates threonine and tyrosine residues on ERK 1 and 2.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 270 mM sucrose, 150 mM NaCl, 50 mM Tris-HCl, 1 mM DTT, 0.1 mM EGTA, pH 7.5

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Allen, L.F., et al. 2003. Semin. Oncol.30, 105.
Schramek, H., et al. 1997. J. Biol. Chem.272, 11426.
Alessandrini, A., et al. 1996. J. Biol. Chem.271, 31612.
One unit is defined as the amount of enzyme that activates ERK1 (0.3 mg/ml) by 1 unit/min using 100 µM ATP at 30°C. One unit ERK1 activity is defined as that amount of enzyme required to transfer 1 pmol phosphate to myelin basic protein, in the presence of 125 µM ATP, per min at 30°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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