Skip to Content
Merck

Key Documents

View All Documentation

480708

α2-3,6-Neuraminidase, Clostridium perfringens, Recombinant, E. coli

Synonym(s):

α2-3,6-Neuraminidase, Clostridium perfringens, Recombinant, E. coli, Acetylneuraminyl Hydrolase, Sialidase

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

CAS Number:
9001-67-6
EC Number:
3.2.1.18 (BRENDA, IUBMB)
NACRES:
NA.54
UNSPSC Code:
12352202
MDL number:
MFCD00149915
Specific activity:
≥10 units/mL, ≥250 units/mg protein
Recombinant:
expressed in E. coli
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

recombinant

expressed in E. coli

Quality Level

100

description

RTECS - QQ3450000

product line

EMPROVE® EXPERT

form

liquid

specific activity

≥10 units/mL, ≥250 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

foreign activity

N-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α- and β-mannosidase, proteases, none detected

shipped in

wet ice

storage temp.

2-8°C

General description

Recombinant, Clostridium perfringens α2-3,6-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal α2,3 and α2,6 unbranched sialic acid residues from complex carbohydrates and glycoproteins. This enzyme does not exhibit activity on α2,8 or branched sialic acids.
Note: 1 mU = 1 milliunit.

Application

Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 6.0

Physical form

In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.

Other Notes

One unit is defined as the amount of enzyme that will catalyze the release of 1 µmol of methylumbelliferone from 2ʹ-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic Acid (Cat. No. 474495) per min at 37°C, pH 5.0.
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
Ohta, Y., et al. 1989. J. Biochem (Tokyo) 106, 1086.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

12 - Non Combustible Liquids

Stay compliant with GMP using the Emprove® Program

Accelerate qualification, maintain compliance, and save time with Emprove® Dossiers.

Download Dossiers

Note: you will be brought to EmproveSuite.com. Your username and password are separate from SigmaAldrich.com and may differ.

Register for free and access Emprove® Dossiers within minutes. Upgrade your subscription plan to unlock advanced and customized content in the Emprove® Suite

Not finding what you are looking for? Visit the Emprove® Program Help page.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Airi Mori et al.
International journal of molecular sciences, 21(16) (2020-08-23)
Polysialic acid (polySia/PSA) is an anionic glycan polymer of sialic acid, and it mostly modifies the neural cell adhesion molecule (NCAM) in mammalian brains. Quality and quantity of the polySia of the polySia-NCAM is spatio-temporally regulated in normal brain development
Yi Yang et al.
International journal of molecular sciences, 21(22) (2020-11-19)
Polysialic acid (polySia/PSA) is a linear homopolymer of sialic acid (Sia) that primarily modifies the neural cell adhesion molecule (NCAM) in mammalian brains. PolySia-NCAM not only displays an anti-adhesive function due to the hydration effect, but also possesses a molecule-retaining