481413
Aurora A Kinase, His•Tag®, Human, Recombinant, S. frugiperda
Aurora A Kinase is a full-length, recombinant (2-403) form fused at the N-terminus to a His•Tag sequence and expressed in S. frugiperda using a baculovirus expression system.
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About This Item
UNSPSC Code:
12352202
Quality Level
Assay
≥90% (SDS-PAGE)
form
liquid
specific activity
≥120 unit/μg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−70°C
General description
Full-length, recombinant, human Aurora A kinase (amino acids 2-403) (GenBank Accession: NM_003600) fused at the N-terminus to a His•Tag sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. Aurora A plays an important role in chromosome segregation and cell division.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 100 mM NaCl, 25 mM Tris-HCl, 3 mM DTT, 50% glycerol, 0.05% Tween®-20 Detergent, pH 7.5.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 1 peptide substrate per min at 30°C, pH 7.4.
Ouchi, M., et al. 2004. J. Biol. Chem.279, 19643.
Kunitoku, N., et al. 2003. Dev. Cell5, 853.
Kunitoku, N., et al. 2003. Dev. Cell5, 853.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC
Disclaimer
Toxicity: Regulatory Review (Z)
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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