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539620

Sigma-Aldrich

PP2A Inhibitor II, GST-Fusion, Human, Recombinant, E. coli - Calbiochem

Synonym(s):

Protein Phosphatase 2A Inhibitor II, I₂ PP2A

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About This Item

UNSPSC Code:
12352200

Assay

≥90% (SDS-PAGE)

Quality Level

100

form

liquid

potency

25-30 nM Ki

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−70°C

General description

This product has been discontinued.



Recombinant, human PP2AI I2 fused to GST and expressed in E. coli. Inhibits protein phosphatase 2A (PP2A) potently with Ki ~ 25-30 nM with myelin basic protein, histone H1, and other substrates of PP2A but not with casein as a substrate. Stimulates PP1. Prevents inhibition of E-Cdk-2 by p21Cip1. Associates with B cyclin. Regulates histone binding to DNA, transcriptional activity and chromatin condensation. Undergoes phosphorylation in cells. Highly expressed in some cancers. Associates with HRX leukemia-associated fusion proteins. Occurs as fusion protein in acute undifferentiated leukemia. Does not inhibit PP1, PP2B, PP2C, or pyruvate dehydrogenase.

Biochem/physiol Actions

Cell permeable: no
Primary Target
PP2A
Product does not compete with ATP.
Reversible: no

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM Tris-HCl, 14 mM βME, 1 mM benzamidine, 1 mM EDTA, 0.1 mM PMSF, 10% glycerol, pH 7.4.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Li, M., et al. 1996. Biochemistry35, 6998.
Li, M., et al. 1995. Biochemistry34, 1988.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class Code

10-13 - German Storage Class 10 to 13

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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