All Photos(1)

Key Documents

COA/COQ

View All Documentation

539620

PP2A Inhibitor II, GST-Fusion, Human, Recombinant, E. coli - Calbiochem

Name: PP2A Inhibitor II, GST-Fusion, Human, Recombinant, <i>E. coli</i></sub> - Calbiochem
Brand: MM

Synonym(s):

Protein Phosphatase 2A Inhibitor II, I₂ ^PP2A

Sign Into View Organizational & Contract Pricing

All Photos(1)

About This Item

UNSPSC Code:

12352200

Assay

≥90% (SDS-PAGE)

Quality Level

100

form

liquid

potency

25-30 nM K_i

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−70°C

General description

This product has been discontinued.

Recombinant, human PP2AI I₂ fused to GST and expressed in E. coli. Inhibits protein phosphatase 2A (PP2A) potently with K_i ~ 25-30 nM with myelin basic protein, histone H1, and other substrates of PP2A but not with casein as a substrate. Stimulates PP1. Prevents inhibition of E-Cdk-2 by p21^Cip1. Associates with B cyclin. Regulates histone binding to DNA, transcriptional activity and chromatin condensation. Undergoes phosphorylation in cells. Highly expressed in some cancers. Associates with HRX leukemia-associated fusion proteins. Occurs as fusion protein in acute undifferentiated leukemia. Does not inhibit PP1, PP2B, PP2C, or pyruvate dehydrogenase.

Biochem/physiol Actions

Cell permeable: no

Primary Target
PP2A

Product does not compete with ATP.

Reversible: no

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM Tris-HCl, 14 mM βME, 1 mM benzamidine, 1 mM EDTA, 0.1 mM PMSF, 10% glycerol, pH 7.4.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Li, M., et al. 1996. Biochemistry35, 6998.
Li, M., et al. 1995. Biochemistry34, 1988.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class Code

10-13 - German Storage Class 10 to 13

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service