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539734

PTP, Yersinia enterocolitica, Recombinant, E. coli

Name: PTP, <i>Yersinia enterocolitica</i>, Recombinant, <i>E. coli</i>
Brand: MM

Synonym(s):

YOP, Protein Tyrosine Phosphatase, Yersenia

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About This Item

UNSPSC Code:

12352202

Assay

≥95% (SDS-PAGE)

Quality Level

100

form

liquid

specific activity

≥50,000 U/mg
≥50,000 units/mL

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

foreign activity

Proteases, serine/threonine phosphatases, none detected

shipped in

wet ice

storage temp.

−70°C

General description

This product has been discontinued.

Recombinant, 51 kDa catalytic domain of the Yersinia protein tyrosine phosphatase containing a C235R mutation and expressed in E. coli. Suitable for dephosphorylation of phosphotyrosine residues in proteins.

Physical form

In 100 mM NaCl, 50 mM HEPES, 5 mM DTT, 2 mM EDTA, 0.01% Brij^® 35 Detergent, 50% glycerol, pH 7.0.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

One unit is defined as the amount of enzyme that will hydrolyze 1 nmol of pNPP per min at 30°C, pH 7.2.

Zhang, Z.Y., et al. 1992. J. Biol. Chem.267, 23759.
Gordon, J.A. 1991. Methods Enzymol.201, 477.
Guan, K. and Dixon, J.E. 1990. Science249, 653.

Legal Information

Brij is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class Code

10-13 - German Storage Class 10 to 13

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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