Anti-Sialyl Lewis a Mouse mAb (KM231)

Purified mouse monoclonal antibody. Recognizes the Sialyl Lewis a epitope on glycoproteins ≥1000 kDa.

Recognizes the SLe^a carbohydrate epitope on glycoproteins and glycolipids on cell membranes and in the cytoplasm of breast, colon, ovarian and stomach cancers, and in lung adenocarcinoma.

Sialyl Lewis a [SLe^a; NeuAcα2,3Galβ1,3(Fucα1,4)GlcNAc] is a non-myeloid-specific tetrasaccharide that acts as a minimal ligand for E-selectin (ELAM; endothelial leukocyte adhesion molecule), an endothelial cell-surface glycoprotein that mediates recruitment of neutrophils and monocytes to sites of endothelial inflammation. This monoclonal antibody is specific for the SLe^a epitope found on various cell-surface glycoproteins and glycolipids.

This Anti-Sialyl Lewis a Mouse mAb (KM231) is validated for use in Adhesion Blockade, ELISA, Flow Cytometry, Immunoblotting, Paraffin Sections Radioimmunoassay for the detection of Sialyl Lewis a.

human gastric cancer cells

Adhesion Blockade (see comments)
ELISA (see comments)
Flow Cytometry (10 μg/ml)
Immunoblotting (see comments)
Paraffin Sections (5 μg/ml)
Radioimmunoassay (see comments)

In PBS, protein stabilizer, pH 7.2.

Following initial thaw, aliquot and freeze (-20°C)

Reacts strongly with an antigen in cell membranes and cytoplasm of various cancerous tissues, including lung adeno-carcinomas, and cancers of the stomach, colon, breast, and ovary. Reacts weakly with various normal tissues, including stomach mucosa, bronc