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566415
PhosphoDetect Anti-Smad2 (pSer465/467) Rabbit pAb
liquid, Calbiochem®
Synonym(s):
Anti-Phospho-MADR2
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About This Item
UNSPSC Code:
12352203
biological source
rabbit
Quality Level
antibody product type
primary antibodies
clone
polyclonal
form
liquid
does not contain
preservative
species reactivity
mink, mouse, human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
General description
This product has been discontinued.
Recognizes the ~58 kDa Smad2 protein phosphorylated at Ser465/467.
Rabbit polyclonal antibody purified by protein A followed by immunoaffinity chromatography. Recognizes the ~58 kDa Smad2 protein phosphorylated at Ser465/467.
Immunogen
a synthetic phosphopeptide surrounding the Ser⁴⁶⁵ and Ser⁴⁶⁷ phosphorylation sites of human Smad2
Application
Immunoblotting (1:1000, see comments)
Physical form
In 150 mM NaCl, 10 mM HEPES sodium, 100 µg/ml BSA, 50% glycerol, pH 7.5.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Heldin, C.H., et al. 1997. Nature390, 465.
Wrana, J. and Pawson, T. 1997. Nature388, 28.
Marcias-Silva, M., et al. 1996. Cell87, 1215
Massague, J., et al. 1996. Cell85, 947.
Wrana, J. and Pawson, T. 1997. Nature388, 28.
Marcias-Silva, M., et al. 1996. Cell87, 1215
Massague, J., et al. 1996. Cell85, 947.
The immunogen seqeunce is conserved in chicken, human, mouse, rat, Xenopus, Zebrafish, and Drosophila. Does not cross-react with other Smad related proteins. For immunoblotting, incubate with antibody diluted in 1x TBS, 0.1% Tween®-20 detergent, and 5% dry milk overnight at 4°C with gentle shaking. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class Code
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
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