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Key Documents

569600

Sigma-Aldrich

Stem Cell Factor, Human, Recombinant, E. coli

Synonym(s):

rhSCF

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About This Item

UNSPSC Code:
12352202

Assay

≥95% (SDS-PAGE)

Quality Level

form

lyophilized

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

≤0.1 ng/μg Endotoxin (ng/μg SCF)

shipped in

ambient

storage temp.

−70°C

General description

Recombinant, human stem, cell factor expressed in E. coli. Hematopoietic growth factor that stimulates the growth of cells of multiple lineage.
Recombinant, human stem, cell factor expressed in E. coli. Hematopoietic growth factor that stimulates the growth of cells of multiple lineages.

Biochem/physiol Actions

ED₅₀ = 2.5-5.0 ng/ml as determined by the dose-dependent proliferation of human MO-7e cells.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute with sterile DI H₂O to 0.1-1.0 mg/ml. Further dilutions should be made in low endotoxin medium or buffer solution containing FBS or tissue culture grade BSA.

Other Notes

McNiece, I.K., et al. 1994. Leuk. Lymphoma 15, 405.
Huang, E., et al. 1990. Cell63, 225.
Martin, F.H., et al. 1990. Cell63, 203.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class Code

10-13 - German Storage Class 10 to 13


Certificates of Analysis (COA)

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