TGF-β1, Human Platelets

Native transforming growth factor-β1 from human platelets. Promotes apoptosis in resting human B lymphocytes, glioma cells, and trigeminal neurinomal cells.

Native transforming growth factor-β1 from human platelets. Promotes apoptosis in resting human B lymphocytes, glioma cells, and trigerminal neurinomal cells.

ED₅₀ = 40-10 pg/ml as measured by the inhibition of IL-4-dependant proliferation of mouse HT-2 cells using resazurin dye as an indicator of cell proliferation.

Lyophilized from a sterile-filtered solution of 50 µg BSA/µg TGF-β1, 35% acetonitrile, 0.1% TFA.

Following reconstitution, store in the refrigerator (4°C) for short-term storage or freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 months at 4°C or up to 3 months at -20°C..

Prepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitute to a concentration ≥10 µg/ml in sterile 4 mM HCl containing ≥0.1% HSA or BSA.

Lomo, J., et al. 1995. J. Immunol.154, 1634.
Marushige, K. and Marushige, Y. 1995. Anticancer Res.14, 2419.
Bermundez, L.E. 1993. J. Immunol.150, 1838.
Hamilton, J.A., et al. 1993. J. Immunol.150, 1496.
Reilly, J.T., et al. 1993. Br. J. Haematol.83, 58.
Sporn, M.B. and Roberts, A.B. 1993. Growth Factors8, 1.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)