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HBNMAG-51K

Millipore

MILLIPLEX® Human Bone Magnetic Bead Panel - Bone Metabolism Multiplex Assay

The analytes available for this multiplex kit are: ACTH, DKK-1, FGF-23, IL-1β, IL-6, Insulin, Leptin, Osteocalcin, OPN - Osteopontin, Osteoprotegerin, PTH, SOST, TNF-α.

Synonym(s):

human bone metabolic biomarkers immunoassay panel, luminex human bone metabolism multiplex assay, millipore human bone metabolic biomarkers multiplex kit

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84

Quality Level

200

species reactivity

human

manufacturer/tradename

Milliplex®

assay range

accuracy: 82-97%
standard curve range: 0.24-1,000 pg/mL
(TNFα)
standard curve range: 1-3,000 pg/mL
(IL-1β)
standard curve range: 1-6,000 pg/mL
(ACTH & IL-6)
standard curve range: 146-600,000 pg/mL
(OC)
standard curve range: 24-100,000 pg/mL
(SOST)
standard curve range: 37-150,000 pg/mL
(FGF-23)
standard curve range: 49-200,000 pg/mL
(Leptin)
standard curve range: 5-20,000 pg/mL
(DKK-1)
standard curve range: 5-20,000 pg/mL
(PTH)
standard curve range: 61-250,000 pg/mL
(Insulin)
standard curve range: 7-30,000 pg/mL
(OPG)
standard curve range: 98-400,000 pg/mL
(OPN)

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

General description

Bone metabolism is the dynamic process of ongoing bone deposition and resorption, controlled by osteoblasts, osteocytes, and osteoclasts. While osteoblasts and osteocytes (osteoblasts surrounded by matrix) are responsible for bone deposition, osteoclasts are responsible for bone resorption. Both are required to maintain bone structure, as well as an adequate supply of calcium. To maintain this metabolic balance these cells rely on complex signaling pathways involving hormones and cytokines to achieve the appropriate rates of growth and differentiation. The disruption of bone metabolism results in such disease as osteoporosis, osteoarthritis, rheumatoid arthritis, chronic kidney disease and bone metastases.

MILLIPLEX® Human Bone Magnetic Bead kit is to be used for the simultaneous quantification of any or all of the following analytics in human plasma, serum or tissue/cell lysate and culture supernatant samples: Adrenocorticotropic Hormone (ACTH), Dickkopf-1 (DKK1), IL-6, Insulin, Leptin, TNFα, Osteopotegrin (OPG), Osteocalcin (OC), Osteopontin (OPN), Sclerostin (SOST), IL-1β, Parathyroid Hormone (PTH) and FGF-23. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Bone

Application

  • Analytes: ACTH, DKK-1, FGF-23, IL-1β, IL-6, Insulin, Leptin, Osteocalcin (OC), Osteopontin (OPN), Osteoprotegerin (OPG), PTH, Sclerostin (SOST), TNFα
  • Recommended Sample Type: Human serum, plasma, and cell/tissue culture supernatants or extracts
  • Recommended Sample Dilution: 25 μL per well of d1:2 diluted serum or plasma; cell/tissue culture samples may be used neat or diluted in appropriate control medium
  • Assay Run Time: Overnight (16–18 hours) at 2-8°C or 2 hours at room temperature (20-25°C)
  • Research Category: Metabolism
  • Research Subcategory: Bone

Biochem/physiol Actions

Cross Reactivty
No cross reactivity between the antibodies for an analyte in this panel were observed.

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Other Notes

Please contact Technical Service for linearity of dilution.
Replaces: HBN1B-51K
Sensitivity: See kit protocol for individual analytes sensitivities.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Certificates of Analysis (COA)

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Chiara Giannasi et al.
International journal of medical sciences, 16(1), 23-32 (2019-01-22)
Bisphosphonates (BPs) represent the first-line treatment for a wide array of bone disorders. Despite their well-known action on osteoclasts, the effects they induce on osteoblasts are still unclear. In order to shed light on this aspect we evaluated the impact
Fawaz Y Azizieh et al.
Biomarker insights, 14, 1177271919843825-1177271919843825 (2019-08-28)
Receptor activator of nuclear factor κB ligand (RANKL), osteoprotegerin (OPG), and oxidative stress markers are suggested to contribute to bone loss in osteoporosis that occurs in menopause. However, the association between these markers and bone mineral density (BMD) is controversial.
Sharon M Moe et al.
Circulation, 132(1), 27-39 (2015-06-11)
Patients with kidney disease have disordered bone and mineral metabolism, including elevated serum concentrations of fibroblast growth factor-23 (FGF23). These elevated concentrations are associated with cardiovascular and all-cause mortality. The objective was to determine the effects of the calcimimetic cinacalcet
Edward R Smith et al.
Scientific reports, 7(1), 5686-5686 (2017-07-20)
Calciprotein particles, nanoscale aggregates of insoluble mineral and binding proteins, have emerged as potential mediators of phosphate toxicity in patients with Chronic Kidney Disease. Although existing immunochemical methods for their detection have provided compelling data, these approaches are indirect, lack
Tsang-Hai Huang et al.
Journal of musculoskeletal & neuronal interactions, 20(2), 206-215 (2020-06-03)
The present study determined time-course changes in plasma bone-specific and -related markers following a bout of maximal eccentric contractions (MaxEC) of bilateral knee extensors (KE) and flexors (KF). Sedentary young men (n=30) performed a bout of 10 sets of 10
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Global Trade Item Number

SKUGTIN
HBNMAG-51K-1104053252799990
HBNMAG-51K-1304053252800016
HBNMAG-51K-0504053252799938
HBNMAG-51K-0604053252799945
HBNMAG-51K-0304053252799914
HBNMAG-51K-0804053252799969
HBNMAG-51K-0904053252799976
HBNMAG-51K-1004053252799983
HBNMAG-51K-1204053252800009
HBNMAG-51K-0704053252799952
HBNMAG-51K-0404053252799921
HBNMAG-51K-0204053252799907
HBNMAG-51K-0104053252799891

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