Skip to Content
Merck

HCD8MAG-15K

MILLIPLEX® Human CD8+ T Cell Panel

Configurable Human CD8+ T Cell 17-Plex Panel

Synonym(s):

Human CD8 T cell cytokine panel, Human cytokine multiplex kit, Luminex® human cytokine immunoassay, Millipore human cytokine panel

Sign In to View Organizational & Contract Pricing.

About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
eCl@ss:
32161000

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

MILLIPLEX® Human CD8+ T Cell Panel, Configurable Human CD8+ T Cell 17-Plex Panel

species reactivity

human

manufacturer/tradename

Milliplex®

Quality Level

200

packaging

1 ea of

assay range

standard curve range: 0.4-1,500 pg/mL
(IL-6)
standard curve range: 0.5-2,000 pg/mL
(TNFα)
standard curve range: 1-3,500 pg/mL
(MIP-1α)
standard curve range: 1-5,000 pg/mL
(Granzyme B)
standard curve range: 1-5,000 pg/mL
(IFNγ)
standard curve range: 1-5,000 pg/mL
(IL-5)
standard curve range: 10-50,000 pg/mL
(Perforin)
standard curve range: 10-50,000 pg/mL
(sFasL)
standard curve range: 2-10,000 pg/mL
(IL-4)
standard curve range: 2-10,000 pg/mL
(sCD137)
standard curve range: 2-7,500 pg/mL
(IL-13)
standard curve range: 2-7,500 pg/mL
(IL-2)
standard curve range: 20-100,000 pg/mL
(Granzyme A)
standard curve range: 4-15,000 pg/mL
(GM-CSF)
standard curve range: 400-1,650,000 pg/mL
(sFas)
standard curve range: 5-20,000 pg/mL
(IL-10)
standard curve range: 7-30,000 pg/mL
(MIP-1β)

technique(s)

multiplexing: suitable

input

serum plasma (Cell culture, cell culture supernatants)

detection method

fluorometric (Luminex® xMAP® technology)

shipped in

wet ice

storage temp.

2-8°C

Related Categories

Biochem/physiol Actions

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Preparation Note

Recommended storage for kit components is 2 - 8°C.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

For research use only. Not for use in diagnostic procedures.Label License/Sticker for Assay Product:By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

Features and Benefits

  • Enhanced Understanding of Immune Response: The ability to measure multiple cytokines and chemokines associated with CD8+ T cell activity aids researchers in understanding the immune response in various conditions, including infections and cancers. Streamlined.
  • Research Process: The overnight run time and simple sample preparation facilitate a more efficient research workflow, allowing for quicker data analysis and decision-making.
  • Configurable Options for Diverse Research Needs: The ability to configure the panel ensures that researchers can adapt the kit to their specific study requirements, making it a versatile tool in immunology research.
  • Conservation of Valuable Samples: By quantifying multiple analytes from a single sample, researchers can conserve precious sample volume, which is especially important in studies involving limited or difficult-to-obtain samples.
  • Comprehensive Analyte Detection: The panel includes 15 key analytes relevant to CD8+ T cell function and activity, such as GM-CSF, IFNγ, IL-2, Granzyme A, and Perforin, allowing for a detailed immune response analysis.

General description

CD8+ T cells (also known as cytotoxic T cells, cytolytic T cells and killer T cells) belong to a sub-group of T cells capable of inducing the death of infected somatic or tumor cells. T cells that bind weakly to Major Histocompatibility Complex (MHC) self-antigens are positively selected into single-positive CD4+ or CD8+ T cells in the thymus. Those CD8+ T cells that survive and mature after activation become cytotoxic cells, expressing T-cell receptors (TCRs) capable of recognizing specific antigenic peptides bound to Class I MHC molecules and the glycoprotein CD8. Affinity between the CD8 protein and the MHC molecule helps to keep the cytotoxic T cell and target closely bound during antigen specific activation. Once activated, CD8+ T cells are generally classified as having a predefined cytotoxic role within the immune system and undergo IL-2 induced clonal expansion, which increases the number of cells specific for the target antigen. The CD8+ T cells then travel throughout the body in search of antigen-positive somatic/tumor cells.

Application

MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze up to 17 analytes in human serum, plasma, and cell culture supernatants.

Analytes included: GM-CSF, sCD137, IFNγ, sFas, sFasL, Granzyme A, Granzyme B, IL-2, IL-4, IL-5, IL-6, IL-10, IL-13, MIP-1α, MIP-1β, TNF-α, Perforin

Assay Characteristics: Refer to kit protocol for assay cross-reactivity, sensitivity, precision, and accuracy.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

target_organs

Respiratory Tract

Storage Class

10 - Combustible liquids

wgk

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Wiebke Naujoks et al.
Frontiers in immunology, 11, 573200-573200 (2020-10-27)
Obesity is associated with an increased risk for several cancer types and an altered phenotype and functionality of natural killer (NK) cells. This study aimed to investigate the association of overweight and obesity with NK cell functions and receptor expression
Chaoxu Zhang et al.
Frontiers in oncology, 10, 760-760 (2020-06-13)
Anti-PD-1 therapy has been approved for cancer treatment. However, the response rate is unsatisfactory. The expression of PD-L1 in tumor tissues is unreliable to predict the treatment response. Recent studies have suggested that exosomal PD-L1 not only exerts immunosuppressive effects
Arturo Panduro et al.
Clinical liver disease, 19(2), 41-48 (2022-03-22)
Content available: Author Interview and Audio Recording.
Antti Hurme et al.
Frontiers in immunology, 13, 869990-869990 (2022-05-10)
The emergence of novel variants of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has made it more difficult to prevent the virus from spreading despite available vaccines. Reports of breakthrough infections and decreased capacity of antibodies to neutralize variants
Hassen Kared et al.
Frontiers in immunology, 9, 686-686 (2018-05-08)
Repetitive stimulation by persistent pathogens such as human cytomegalovirus (HCMV) or human immunodeficiency virus (HIV) induces the differentiation of natural killer (NK) cells. This maturation pathway is characterized by the acquisition of phenotypic markers, CD2, CD57, and NKG2C, and effector
View All Related Papers

Related Content

Immunology Multiplex Assays | MILLIPLEX® Assays

Immunology multiplex assays, like MILLIPLEX® assays, allow researchers to investigate more immune biomarkers simultaneously, saving time and resources.

Overview of Luminex® Multiplex Assay Technology | MILLIPLEX® Multiplex Assays

Discover the benefits of MILLIPLEX® multiplex assays, based on Luminex® xMAP® multiplex assay technology, that provide consistent, high-quality results and see how these multiplex biomarker Luminex® assays are being used to advance research.

Multiplexing Inflammation Signaling Pathways | MILLIPLEX® Assays

See how multiplexing the inflammation signaling pathway with MILLIPLEX® inflammation assays or cell signaling assays can help researchers bridge the gap between immunology and cell signaling, including investigating T cell signaling, Th Cell differentiation, inflammatory response signaling, and sepsis signaling.

The Power of Biomarker Analysis
See All

Global Trade Item Number

SKUGTIN
HCD8MAG-15K-0404053252830334
HCD8MAG-15K-08C04055977106527
HCD8MAG-15K-12C04055977099652
HCD8MAG-15K-03C04055977104349
HCD8MAG-15K-1404053252830433
HCD8MAG-15K-13C04055977099669
HCD8MAG-15K-0504053252830341
HCD8MAG-15K-1704053252830464
HCD8MAG-15K-1504053252830440
HCD8MAG-15K-1204053252830419
HCD8MAG-15K-09C04055977104363
HCD8MAG-15K-0604053252830358
HCD8MAG-15K-1604053252830457
HCD8MAG-15K-1104053252830402
HCD8MAG-15K-04C04055977099621
HCD8MAG-15K-14C04055977104387
HCD8MAG-15K-01C04055977104332
HCD8MAG-15K-11C04055977104370
HCD8MAG-15K-07C04055977099645
HCD8MAG-15K-17C04055977099676
HCD8MAG-15K-02C04055977106510
HCD8MAG-15K-1004053252830396
HCD8MAG-15K-0304053252830327
HCD8MAG-15K-10C04055977108194
HCD8MAG-15K-1304053252830426

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service