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NB21

Sigma-Aldrich

Anti-N-Ethylmaleimide-Sensitive Factor Mouse mAb (NSF-1)

liquid, clone NSF-1, Calbiochem®

Synonym(s):

Anti-NSF

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

100

antibody product type

primary antibodies

clone

NSF-1, monoclonal

form

liquid

contains

3 mM sodium azide as preservative

species reactivity

human, rat

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

General description

This product has been discontinued.



Recognizes the ~76 kDa NSF-1 protein in human fibroblasts, MDBK, and PC12 cells.

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myeloma cells. Recognizes the ~76 kDa NSF-1 protein.

Immunogen

Human
recombinant, human N-ethylmaleimide-senstive factor

Application

ELISA (1:2000)

Immunoblotting (1:2000)

Immunoprecipitation (1:500)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 20 mM sodium phosphate, 50% glycerol, pH 7.5.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Human or rat fibroblasts, MDBK cells, or PC12 cells

Other Notes

Sutton, R.B., et al. 1998. Nature395, 347.
Lemons, P.P., et al. 1997. Blood90, 1490.
Edelmann, L., et al. 1995. EMBO J.14, 224.
Südhof, T.C. 1995. Nature375, 645.
Whiteheart, S.W., et al. 1994. J. Cell Biol.126, 945.
This antibody is suitable for ELISA and immunoprecipitation under both native and denaturing conditions. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class Code

10-13 - German Storage Class 10 to 13

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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