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NE1023

Sigma-Aldrich

Anti-Neurofilament H Non-Phosphorylated Mouse mAb (SMI-32)

liquid, clone SMI-32, Calbiochem®

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

100

antibody product type

primary antibodies

clone

SMI-32, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity (predicted by homology)

mammals

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

General description

This product has been discontinued.



We are offering Anti-Neurofilament H Non-Phosphorylated Mouse MAb (SMI-32) (Cat. No. 559844) as a possible alternative. Please read the alternative product documentation carefully and contact technical service if you need additional information.






Recognizes the non-phosphorylated ~180 kDa-200 kDa neurofilament H protein in rat central nervous system (CNS) cytoskeletal preparations.

Mouse monoclonal antibody supplied as undiluted ascites. Recognizes the ~180-200 kDa non-phosphorylated neurofilament H protein.

Immunogen

Rat
homogenized hypothalami from Fischer 344 rat brain

Application


ELISA (1:1000)
Frozen Sections (1:1000, see comments)
Immunoblotting (1:1000, see comments)
Immunocytochemistry (1:1000, see comments)
Paraffin Sections (1:1000, heat pre-treatment required, see comments)

Physical form

Undiluted ascites.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Rat brain or central nervous system cytoskeletal preparations

Other Notes

Only recognizes non-phosphorylated neurofilament H. By immunocytochemistry this antibody stains neuronal cell bodies, dendrites, and some thick axons in the central and peripheral nervous systems, but does not stain thin axons or other cells and tissues. Tissues and cultured cells can be fixed in a variety of paraformaldehyde- or formaldehye-containing fixatives, such as Bouin′s fixative. Antibody reactivity is poor in glutaraldehyde/paraformaldehyde-fixed samples. For staining formalin-fixed, paraffin sections it is recommended that de-paraffinized tissue be autoclaved in dH2O for 10 min or boiled in Tris-buffered saline, pH 9.0, in a microwave for 15 min. Trypsin pre-treatment abolishes antibody binding to the epitope. Post-fixation in cold methanol or methanol/hydrogen peroxide facilitates access of the antibody to the neurons in frozen sections or thick sections fixed in 4% paraformaldehyde and in cultured cells. By immunoblotting this antibody detects two bands, ~180 and ~200 kDa, which merge into a single NFH line on two-dimensional gels. Antibody should be titrated for optimal results in individual systems.
Trapp, B.D., et al. 1998. N. Engl. J. Med.338, 278.
King, C.E., et al. 1997. Neuroreport.8, 1663.
Campbell, M.J., et al. 1991. Brain Res.539, 133.
Campbell, M.J., et al. 1989. J. Comp. Neurol.282, 191.
Sternberger, L.A., et al. 1983. Proc. Natl. Acad. Sci. USA80, 6126.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class Code

10-13 - German Storage Class 10 to 13

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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