Key Documents
PC522
Anti-Adenine Nucleotide Translocase-1 (Ab-1) (2-11) Rabbit pAb
liquid, Calbiochem®
Synonym(s):
Anti-ANT-1
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About This Item
UNSPSC Code:
12352203
biological source
rabbit
antibody product type
primary antibodies
clone
polyclonal
form
liquid
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
dry ice
storage temp.
−20°C
General description
Anti-Adenine Nucleotide Translocase-1 (Ab-1) (2-11), rabbit polyclonal, recognizes ~35 kDa ANT-1 in doxorubicin-treated MCF-7 cells. It is validated for WB, immunofluorescence, and paraffin sections.
Purified rabbit polyclonal antibody. Recognizes the ~35 kDa ANT-1 protein.
Recognizes the ~35 kDa ANT-1 protein in doxorubicin-treated MCF-7 cells or breast carcinoma tissue.Antibody Target Gene Symbol: ANK3 Target Synonym: 2900054D09RIK, AI314020, AnkG, Ankyrin-3, ANKYRIN-G, FLJ45464, MGC14049 Entrez Gene Name: ankyrin 3, node of Ranvier (ankyrin G) Hu Entrez ID: 288 (Related Antibodies: NB20, AP1034, PC523, AP1057) Mu Entrez ID: 11735 Rat Entrez ID: 361833
Immunogen
Human
a synthetic peptide corresponding to amino acids 2-11 of human ANT-1
Application
Immunoblotting (2.5 g/ml, chemiluminescence)
Immunofluorescence (1 g/ml)
Paraffin Sections (1 g/ml, heat pre-treatment required)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 50 mM sodium phosphate buffer, 50% glycerol.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
doxorubicin-treated MCF7 cells, or breast carcinoma tissue
doxorubicin-treated MCF7 cells, or breast carcinoma tissue
Other Notes
Bauer, M.K.A., et al. 1999. J. Cell Biol.147, 1493.
Marzo, I, et al. 1998. Science281, 2027.
Marzo, I, et al. 1998. J. Exp. Med.187, 1261.
Zoratti, M. and Szabo, I. 1995. Biochim. Biophys. Acta1231, 139.
Klingenberg, M. 1993. J. Bioenerg. Biomembr.25, 447.
Riccio, P., et al. 1975. FEBS Lett.56, 133.
Marzo, I, et al. 1998. Science281, 2027.
Marzo, I, et al. 1998. J. Exp. Med.187, 1261.
Zoratti, M. and Szabo, I. 1995. Biochim. Biophys. Acta1231, 139.
Klingenberg, M. 1993. J. Bioenerg. Biomembr.25, 447.
Riccio, P., et al. 1975. FEBS Lett.56, 133.
MCF7 cells treated with doxorubicin at 0.2 µg/ml for 24 h can be used as positive control for immunoblotting. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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