B0149

Brilliant Blue R

Name: Brilliant Blue R
Brand: SIAL

Dye content ~50 %, Powder

Synonym(s):

Acid Blue 83, Brilliant indocyanin 6B, Coomassie Brilliant Blue R

Select a Size

All Photos(5)

About This Item

Empirical Formula (Hill Notation):

C₄₅H₄₄N₃NaO₇S₂

CAS Number:

6104-59-2

Molecular Weight:

825.97

UNSPSC Code:

12171500

NACRES:

NA.47

PubChem Substance ID:

24891505

EC Number:

228-060-5

MDL number:

MFCD00041762

Colour Index Number:

42660

Beilstein/REAXYS Number:

5718025

Product Name

Brilliant Blue R, Dye content ~50 %, Technical grade

form

powder

InChI key

NKLPQNGYXWVELD-UHFFFAOYSA-M

InChI

1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1

SMILES string

[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=C/C(C=C5)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1

composition

Dye content, ~50%

technique(s)

microbe id | staining: suitable

solubility

H₂O: 10 mg/mL

suitability

suitable for detection of protein bands following electrophoresis.

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

Looking for similar products? Visit Product Comparison Guide

Related Categories

Clinical & Diagnostics

Hematology Stains

Histology Stains

Tissue Diagnostics

Application

Brilliant Blue R has been widely used for protein band detection after SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis).Its other applications include:

as a protein stain on epoxy-embedded tissue sections
for quantitative micro densitometry of protein in sections
for staining chromatids, actinin microfilaments, developing neurites, and sperm acrosomes.

For detection of protein bands following electrophoresis.

General description

Brilliant Blue R (C.l. 42660) is a triaminotriarylmethane dye with two amino groups being benzylated and one phenylated. It is also known as brilliant indocyanine 6B and coomassie blue R250.

Legal Information

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

0000507765

0000420376

0000420377

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Role of matrix metalloproteinase-2 in the cardioprotective effect of ischaemic postconditioning.

Donato M, et al.

Experimental Physiology, 95, 274-281 (2010)

Effect of Flammulina velutipes on spent-hen breast meat tenderization.

Kang GH, et al.

Poultry Science, 91, 232-236 (2012)

The strategic combination of trastuzumab emtansine with oncolytic rhabdoviruses leads to therapeutic synergy.

Rozanne Arulanandam et al.

Communications biology, 3(1), 254-254 (2020-05-24)

We have demonstrated that microtubule destabilizing agents (MDAs) can sensitize tumors to oncolytic vesicular stomatitis virus (VSVΔ51) in various preclinical models of cancer. The clinically approved T-DM1 (Kadcyla®) is an antibody-drug conjugate consisting of HER2-targeting trastuzumab linked to the potent

Transcription-terminating mutation in telethonin causing autosomal recessive muscular dystrophy type 2G in a European patient.

Montse Olivé et al.

Neuromuscular disorders : NMD, 18(12), 929-933 (2008-10-25)

A 27-year-old woman of Moldavian origin presented at the age of 15 with progressive proximal limb weakness and painful cramps in her calf muscles. Clinical examination revealed prominent muscle weakness in proximal muscles of the lower extremities and distal anterior

Peptide mass fingerprint sequence coverage from differently stained proteins on two-dimensional electrophoresis patterns by matrix assisted laser desorption/ionization-mass spectrometry (MALDI-MS).

C Scheler et al.

Electrophoresis, 19(6), 918-927 (1998-06-25)

Identification of proteins separated by two-dimensional electrophoresis (2-DE) is a necessary task to overcome the purely descriptive character of 2-DE and a prerequisite to the construction of 2-DE databases in proteome projects. Matrix assisted laser desorption/ionization-mass spectrometry (MALDI-MS) has a

View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service