B6529

Brilliant Blue R Staining Solution

Name: Brilliant Blue R Staining Solution
Brand: SIAL

ethanol solution

Synonym(s):

Brilliant Blue R, Acid Blue 83, Brilliant indocyanin 6B, Coomassie Brilliant Blue R

Select a Size

All Photos(1)

About This Item

Empirical Formula (Hill Notation):

C₄₅H₄₄N₃NaO₇S₂

CAS Number:

6104-59-2

Molecular Weight:

825.97

UNSPSC Code:

12171500

NACRES:

NA.47

PubChem Substance ID:

24891914

MDL number:

MFCD00041762

Beilstein/REAXYS Number:

5718025

Colour Index Number:

42660

Product Name

Brilliant Blue R Staining Solution, suitable for (for immunoelectrophoresis protein staining)

InChI

1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1

SMILES string

[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=C/C(C=C5)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1

InChI key

NKLPQNGYXWVELD-UHFFFAOYSA-M

form

liquid

technique(s)

microbe id | staining: suitable

color

dark blue

suitability

suitable for (for immunoelectrophoresis protein staining)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

Looking for similar products? Visit Product Comparison Guide

Related Categories

Clinical & Diagnostics

Hematology Stains

Histology Stains

Tissue Diagnostics

Analysis Note

Tested for suitability on agarose gels following immunoelectrophoresis.

Application

Brilliant Blue R staining solution is especially designed for use in staining protein in agarose gels following immunoelectrophoresis and Ouchterlony gels. The stain contains ethanol and acetic acid so gels do not require fixing prior to staining. The immunoelectrophoresis or Ouchterlony gel is first washed with water and saline to remove non-precipitated proteins and then dried. The gel is then immersed in staining solution for 30 min and destained with 10% acetic acid.

For detection of protein bands following electrophoresis.

Other Notes

0.5% (w/v) Brilliant Blue R, 45% (v/v) ethanol and 10% (v/v) acetic acid.

Legal Information

pictograms

GHS02,GHS07

signalword

Warning

hcodes

H226,H315,H319

pcodes

P210 - P233 - P240 - P241 - P303 + P361 + P353 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 3 - Skin Irrit. 2

Storage Class

3 - Flammable liquids

wgk

WGK 2

flash_point_f

81.0 °F - closed cup

flash_point_c

27.2 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Proteomics profiling of plasma exosomes in epithelial ovarian cancer: A potential role in the coagulation cascade, diagnosis and prognosis.

Wei Zhang et al.

International journal of oncology, 54(5), 1719-1733 (2019-03-14)

Ovarian cancer remains the most lethal type of cancer among all gynecological malignancies. The majority of patients are diagnosed with ovarian cancer at the late stages of the disease. Therefore, there exists an imperative need for the development of early

Destaining of Coomassie Brilliant Blue R-250-stained polyacrylamide gels with sodium chloride solutions.

G Sreeramulu et al.

Electrophoresis, 16(3), 362-365 (1995-03-01)

A novel method for destaining of polyacrylamide gels, stained with Coomassie Brilliant Blue R-250, is described, based on the use of 0.5 M NaCl in water as the destainer, requiring only 2-3 h. Concentrated (> 2 M) or dilute (<

Enhanced stability and decolorization of Coomassie Brilliant Blue R-250 by dextran aldehyde-modified horseradish peroxidase.

Melda Altikatoglu et al.

Artificial cells, blood substitutes, and immobilization biotechnology, 39(3), 185-190 (2010-12-02)

Horseradish peroxidase (EC 1.11.1.7) was chemically modified by periodate-activated dextran. The activities of free and modified enzyme against organic-aqueous interface and some chemicals were determined. Modified HRP remained fully active in the presence of organic solvent for 4 h. However

Recovery of single-band proteins from polyacrylamide gels by using electrophoresis in solutions with different ionic strengths and specific weights.

Ding-Gan Liu

Analytical biochemistry, 339(2), 351-352 (2005-03-31)

Decolorization of malachite green by cytochrome c in the mitochondria of the fungus Cunninghamella elegans.

Yong-Hak Kim et al.

Archives of biochemistry and biophysics, 494(2), 159-165 (2009-12-01)

We studied the decolorization of malachite green (MG) by the fungus Cunninghamella elegans. The mitochondrial activity for MG reduction was increased with a simultaneous increase of a 9-kDa protein, called CeCyt. The presence of cytochrome c in CeCyt protein was

View All Related Papers

Articles

Protein Staining Reagents Selection Guide

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.